Identification and application of a strong bidirectional acmN2p promoter from actinomycin D-producing streptomycetes  

作　　者：Sainan Li Danfeng Tang Xu Zhao Manxiang Zhu Xiangcheng Zhu Yanwen Duan Yong Huang 

机构地区：[1]Xiangya International Academy of Translational Medicine,Central South University,Changsha 410013,China [2]Institute of Health and Medicine,Hefei Comprehensive National Science Center,Hefei 230093,China [3]Hunan Engineering Research Center of Combinatorial Biosynthesis and Natural Product Drug Discovery,Changsha 410011,China [4]National Engineering Research Center of Combinatorial Biosynthesis for Drug Discovery,Changsha 410011,China

出　　处：《Engineering Microbiology》2024年第1期108-114,共7页工程微生物学（英文）

基　　金：supported in parts by National Natural Science Foundation of China grants(82173688 and 82373772);The science and technology innovation Program of Hunan Province(2021RC4067);a research fund from Institute of Health and Medicine(IHM),Hefei Comprehensive National Science Center(to Y.H.);the Chinese Ministry of Education 111 Project(BP0820034)(to Y.D.);the Fundamental Research Funds for the Central Universities of Central South University 2020zzts247(to D.T.).

摘　　要：Natural product biosynthesis is controlled at multiple levels.Characterization of naturally occurring promoters has facilitated the study of the synthetic biology of natural products.Herein,we report the discovery of two highyield actinomycin D(ActD)-producing streptomycetes and the identification of a strong bidirectional acmN2p promoter from the ActD gene clusters and its application in heterologous expression of three core genes involved in the bacterial alkaloid bohemamine biosynthesis,providing a good example for identification of new promoters for synthetic biological applications.

关 键 词：Actinomycin D Bidirectional promoter Bohemamine STREPTOMYCES 

分 类 号：Q93[生物学—微生物学]