Gut pathobiome mediates behavioral and developmental disorders in biotoxin-exposed amphibians  

作　　者：Qianqian Pan Tianxing Lv Haorong Xu Hongda Fang Meng Li Jiaping Zhu Yue Wang Xiaoyan Fan Ping Xu Xiuguo Wang Qiangwei Wang Haruna Matsumoto Mengcen Wang 

机构地区：[1]Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Hangzhou,310058,China [2]Institute of Pesticide and Environmental Toxicology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310058,China [3]Institution of Tea Science,Zhejiang University,Hangzhou,310058,China [4]The Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,266101,China [5]Global Education Program for AgriScience Frontiers,Graduate School of Agriculture,Hokkaido University,Sapporo,Japan

出　　处：《Environmental Science and Ecotechnology》2024年第5期208-218,共11页环境科学与生态技术（英文）

基　　金：supported by the National Natural Science Foundation of China(32122074);the National Key R&D Program of China(2023YFD1400901);the Zhejiang University Global Partnership Fund,and the Fundamental Research Funds for the Central Universities(2021FZZX001-31 and 226-2023-00070).

摘　　要：Emerging evidence suggests a link between alterations in the gut microbiome and adverse health outcomes in the hosts exposed to environmental pollutants.Yet,the causal relationships and underlying mechanisms remain largely undefined.Here we show that exposure to biotoxins can affect gut pathobiome assembly in amphibians,which in turn triggers the toxicity of exogenous pollutants.We used Xenopus laevis as a model in this study.Tadpoles exposed to tropolone demonstrated notable developmental impairments and increased locomotor activity,with a reduction in total length by 4.37%e22.48%and an increase in swimming speed by 49.96%e84.83%.Fusobacterium and Cetobacterium are predominant taxa in the gut pathobiome of tropolone-exposed tadpoles.The tropolone-induced developmental and behavioral disorders in the host were mediated by assembly of the gut pathobiome,leading to transcriptome reprogramming.This study not only advances our understanding of the intricate interactions between environmental pollutants,the gut pathobiome,and host health but also emphasizes the potential of the gut pathobiome in mediating the toxicological effects of environmental contaminants.

关 键 词：Gut microbiome Pathobiome Biotoxin pollution Developmental toxicity Behavioral disorders 

分 类 号：R574[医药卫生—消化系统]