Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates  

作　　者：Yan Zhang Yu Qiu Kai Liu Wenjun Zhong Jianghua Yang Florian Altermatt Xiaowei Zhang 

机构地区：[1]State Key Laboratory of Pollution Control&Resource Reuse,School of the Environment,Nanjing University,Nanjing,210023,China [2]Department of Evolutionary Biology and Environmental Studies,University of Zurich,Zürich,Switzerland [3]Department of Aquatic Ecology,Eawag:Swiss Federal Institute of Aquatic Science and Technology,Dübendorf,Switzerland [4]Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi,214081,China

出　　处：《Environmental Science and Ecotechnology》2024年第5期396-404,共9页环境科学与生态技术（英文）

基　　金：the National Key Research and Development Program of China(2022YFC32021001,2021YFC3201003);support.X.Z.was supported by the Fundamental Research Funds for the Central Universities.F.A.was supported by the University of Zurich Research Priority Program“URPP Global Change and Biodiversity”.Y.Z.thanks the China Scholarship Council(CSC NO.202206190065);supporting the visit of the Swiss Federal Institute of Aquatic Science and Technology(Eawag)and the University of Zurich.

摘　　要：The monitoring and management of aquatic ecosystems depend on precise estimates of biodiversity.Metabarcoding analyses of environmental nucleic acids(eNAs),including environmental DNA(eDNA)and environmental RNA(eRNA),have garnered attention for their cost-effective and non-invasive biomonitoring capabilities.However,the accuracy of biodiversity estimates obtained through eNAs can vary among different organismal groups.Here we evaluate the performance of eDNA and eRNA metabarcoding across nine organismal groups,ranging from bacteria to terrestrial vertebrates,in three crosssections of the Yangtze River,China.We observe robust complementarity between eDNA and eRNA data.The relative detectability of eNAs was notably influenced by major taxonomic groups and organismal sizes,with eDNA providing more robust signals for larger organisms.Both eDNA and eRNA exhibited similar cross-sectional and longitudinal patterns.However,the detectability of larger organisms declined in eRNA metabarcoding,possibly due to differential RNA release and decay among different organismal groups or sizes.While underscoring the potential of eDNA and eRNA in large river biomonitoring,we emphasize the need for differential interpretation of eDNA versus eRNA data.This highlights the importance of careful method selection and interpretation in biomonitoring studies.

关 键 词：Environmental nucleic acids Aquatic biodiversity Organismal size Species detectability 

分 类 号：X171[环境科学与工程—环境科学]