猴头菌-黄芪渣菌质总皂苷的优化提取及抗氧化活性研究  

Optimization extraction and antioxidant activity of total saponins from Hericium erinaceus-Astragalus membranaceus residue mycoplasm

作　　者：宋朝冉 时馨 吴雨龙 江海涛 李盛杰 霍光明 华春 SONG Zhao-ran;SHI Xin;WU Yu-long;JIANG Hai-tao;LI Sheng-jie;HUO Guang-ming;HUA Chun

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京晓庄学院食品科学学院,江苏南京211171 [3]江苏省高校特殊生物质废弃物资源化利用重点建设实验室,江苏南京211171

出　　处：《饲料研究》2024年第17期102-108,共7页Feed Research

基　　金：国家自然科学基金项目(项目编号:21376112);江苏省自然基金面上项目(项目编号:BK2014020929);江苏省高校自然科学研究重大项目(项目编号:20KJA416003);江苏省高校自然科学研究面上项目(项目编号:18KJD180002);南京晓庄学院青年专项(项目编号:2019NXY47)。

摘　　要：试验研究经双向固体发酵的猴头菌-黄芪渣菌质总皂苷(HAMTS)的优化提取方案,并测定其抗氧化活性。采用香草醛法测定发酵前后黄芪渣与HAMTS中总皂苷的含量,采用响应面法结合超声法优化提取HAMTS,并分析HAMTS的还原力及对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)自由基的清除能力。结果显示:发酵后HAMTS含量为10.34 mg/g,提升了63.35%。在最佳提取工艺条件下,即液料比19 mL/g、超声时间16 min、乙醇体积分数74%、超声功率62 W,HAMTS提取量为11.48 mg/g。在HAMTS浓度为10 g/L时,HAMTS的还原力以及DPPH和ABTS+自由基的清除率分别为0.80、96.48%和56.12%。研究表明,试验建立的回归模型可用于预测HAMTS提取量,且HAMTS具有较好的抗氧化活性,具有开发为饲料添加剂的潜力。This study aimed to optimize the extraction process of total saponins from the fungal matrix resulting from the dual solid-state fermentation of Hericium erinaceus-Astragalus membranaceus residue(HAMTS)and to determine its antioxidant activity.The content of total saponins before and after fermentation was measured using the vanillin method.A response surface method combined with ultrasonication was employed to optimize the extraction of HAMTS,and the reducing power as well as the scavenging ability against DPPH and ABTS+free radical of HAMTS were analyzed.The results showed that the content of HAMTS after fermentation was 10.34 mg/g,with an increase of 63.35%.Under the optimal extraction conditions:Liquid-to-solid ratio of 19 mL/g,ultrasonication time of 16 min,ethanol volume fraction of 74%,ultrasonic power of 62 W,the extraction amount of HAMTS was 11.48 mg/g.At a concentration of 10 g/L,the reducing power of HAMTS,as well as the DPPH and ABTS+free radical scavenging rates,were 0.80,96.48%,and 56.12%,respectively.The study indicates that the regression model established in the experiment can be used to predict the extraction amount of HAMTS,and HAMTS exhibits good antioxidant activity,demonstrating the potential for development as a feed additive.

关 键 词：猴头菌-黄芪渣菌质 总皂苷 优化提取 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]