温胆汤通过调控p38 MAPK信号通路干预内淋巴积水形成的研究  

Intervention of decoction for warming the gallbladder on formation of endolymphatic hydrops via regulating p38 MAPK signaling pathway

作　　者：夏晶晶[1] 刘金利 侯小兵[1] 陈明[1] 廖龙燕 花海兵[1] XIA Jingjing;LIU Jinli;HOU Xiaobing;CHEN Ming;LIAO Longyan;HUA Haibing(Jiangyin Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangyin 210046,Jiangsu,China)

机构地区：[1]南京中医药大学江阴附属医院,江苏江阴210046

出　　处：《现代中西医结合杂志》2024年第16期2222-2226,2309,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：南京中医药大学自然科学基金项目(XZR2023080);江阴市中医药学会重点科研项目(Z202204)。

摘　　要：目的基于p38丝裂原活化蛋白激酶(p38 MAPK)信号通路探讨温胆汤对内淋巴积水形成的影响。方法将30只豚鼠随机分为对照组、模型组、低剂量温胆汤组、中剂量温胆汤组、高剂量温胆汤组,每组6只。采用腹腔注射6μg/kg醋酸去氨加压素10 d的方法,对除对照组外的其他组豚鼠建立内淋巴积水模型;第11天开始,低、中、高剂量温胆汤组分别灌胃6.1 g/kg、12.2 g/kg、24.4 g/kg温胆汤颗粒剂,对照组和模型组灌胃等量双蒸水。持续灌胃10 d后处死豚鼠,免疫组化法检测耳蜗组织中炎症因子[白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)]和水通道蛋白2(AQP2)阳性表达情况,qRT-PCR法检测耳蜗组织中AQP2和p38 MAPK mRNA表达情况,Western blot法检测耳蜗组织中p38 MAPK、p-p38 MAPK蛋白表达情况。结果与对照组比较,模型组耳蜗组织中IL-1β、IL-6、AQP2阳性表达吸光度值和AQP2 mRNA相对表达量、p-p38 MAPK/p38 MAPK比值均明显升高(P均<0.05),p38MAPK mRNA相对表达量无明显变化(P>0.05);与模型组比较,温胆汤各组耳蜗组织中IL-1β、IL-6、AQP2阳性表达吸光度值和AQP2 mRNA相对表达量、p-p38 MAPK/p38 MAPK比值均明显降低(P均<0.05),p38MAPK mRNA相对表达量均无明显变化(P均>0.05)。结论温胆汤可以有效改善醋酸去氨加压素诱导的豚鼠内淋巴积水,其机制可能与调控p38 MAPK信号通路和AQP2表达有关。Objective It is to investigate the effect of decoction for warming the gallbladder on formation of endolymphatic hydrops via regulating p38 MAPK signaling pathway.Methods Thirty guinea pigs were randomly divided into control group,model group,low-dose,medium-dose and high-dose groups of decoction for warming the gallbladder,with 6 pigs in each group.The pigs of all groups except for the control group were treated with 6μg/kg of desmopressin acetate by intraperitoneal injection for 10 days to establish models of endolymphatic hydrops.Starting from the 11th day,the ow-dose,medium-dose and high-dose groups of decoction for warming the gallbladder were gavaged with 6.1 g/kg,12.2 g/kg and 24.4 g/kg of decoction for warming the gallbladder,respectively,the control group and model group were gavaged with equal volume of double-distilled water.The guinea pigs were executed after 10 d of continuous gavage,and the positive expressions of inflammatory factors[interleukin-1β(IL-1β),interleukin-6(IL-6)]and aquaporin 2(AQP2)in cochlear tissues were detected by immunohistochemistry,the expressions of AQP2 and p38 MAPK mRNA in cochlear tissues were detected by qRT-PCR,and the expressions of p38 MAPK,p-p38 MAPK proteins in cochlear tissues were detected by Western blot.Results Compared with the control group,the absorbance values of positive expressions of IL-1β,IL-6,AQP2 and relative expressions of AQP2 mRNA in cochlear tissues and p-p38 MAPK/p38 MAPK ratio of the model group were significantly increased(all P<0.05),and there was no significant change in the relative expression of p38MAPK mRNA(P>0.05);compared with the model group,the absorbance values of positive expressions of IL-1β,IL-6,AQP2 and relative expressions of AQP2 mRNA in cochlear tissues and p-p38 MAPK/p38 MAPK ratio of each dose group of decoction for warming the gallbladder were significantly decreased(all P<0.05),and there was no significant change in the relative expression of p38MAPK mRNA(P>0.05).Conclusion Decoction for warming the gallbladder can effect

关 键 词：温胆汤 内淋巴积水 水通道蛋白2 P38丝裂原活化蛋白激酶 

分 类 号：R-332[医药卫生]