Effects of Honeysuckle Chlorogenic Acid on Secretory Enzymes,Lipoxygenase A4,and Biochemical Indicators inModel Mice with Aluminum Induced Alzheimer's Disease  

作　　者：Jialing HUANG Cuijuan HUANG Xiaomian TANG Weiming ZHOU Hanchao FENG Cuiping WEI Oufei HUANG Haipeng ZHANG Song NONG Shuqiu ZHANG Fuyu PAN 

机构地区：[1]Youjiang Medical University for Nationalities,Baise 533000,China [2]Research Department of Guangxi Yunqiu Bio-technoloy Co.,Ltd.,Baise 533000,China [3]Research Center of Baise Qingxing Technology Co.,Ltd.,Baise 533000,China [4]Laboratory for Research on Heavy Metals and Fluoroarsenic Toxic Substances,Baise 533000,China

出　　处：《Medicinal Plant》2024年第5期35-38,43,共5页药用植物（英文）

基　　金：Supported by Baise Science Research and Technology Development Plan Project(20232022);Cuangxi College Students’Innovation and Entrepreneurship Training Program(Recommend National Level2022210599040S).

摘　　要：[Objectives]To explore the effects of chlorogenic acid from honeysuckle on the secretion enzymes,lipoxygenase A4(LXA4),and blood biochemical indicators in mice with aluminum induced Alzheimer's disease(AD).[Methods]Chlorogenic acid was extracted from hon-eysuckle by ultrasound assisted alcohol extraction method.Seventy mice were randomly divided into normal group,model group,and low,me-dium and high dose groups of honeysuckle chlorogenic acid.All the mice in each group except for the normal group were given maltol aluminum by intraperitoneal injection to establish models of aluminum induced AD,continuously injected for 5 d and stopped for 2 d,totally poisoned for 8 weeks.Starting from the 5^(th) week of poisoning,the low,medium and high dose groups of honeysuckle chlorogenic acid were given honeysuck-le chlorogenc acid solution 40,80 and 160 mg/kg by gavage,respectively,while the normal group and the model group were fed with an equal volume of distilled water,all once daily,continuously gavaged until the end of the 8^(th) week.At the end of the experiment,the learning memory ability of the mice was tested by Y-type waler maze,and the number of tests required to reach the learning standard,the number of memory er-rors in 20 tests and the error rate of the mice were recorded.The brains of mice were taken to determine the contents of β-secretase,α-secre-tase,γ-secretase,LXA4 and acetylcholinesterase(AchE)in the homogenates of brain tissues by ELISA,and their blood was taken to deter-mine the biochemical indexes.[Results]Compared with the normal group,the number of learning tests,number of memory errors,error rate and the contents of β-secretase,γ-secretase and AchE in brain tissue of the mice in the model group were all significantly increased(all P<0.05),the contents of LXA4 in brain tissue were significantly decreased(all P<0.05),and the contents of α-secretase did not change significantly(all P>0.05);compared with the model group,the number of learning tests,the number of memory errors,the error rate and t

关 键 词：Alzheimer's disease Chlorogenic acid SECRETASE Lipoxygenase A4 

分 类 号：R285.5[医药卫生—中药学]