红景天苷对小鼠成牙本质细胞系MDPC⁃23增殖、迁移和矿化能力影响的研究  

Study on the effect of salidroside on proliferation,migration and mineralization of mouse odontoblast cell line MDPC⁃23

作　　者：关健 吕晶[1] 高雪峰 金星爱[1] GUAN Jian;LYU Jing;GAO Xuefeng;JIN Xing’ai(Department of Pediatric Stomatology,The First Affiliated Hospital of Harbin Medical University,School of Stomatology,Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院,哈尔滨医科大学口腔医学院儿童口腔科,黑龙江哈尔滨150001

出　　处：《口腔生物医学》2024年第5期265-270,共6页Oral Biomedicine

基　　金：黑龙江省教育厅面上项目(12521348)。

摘　　要：目的:探究红景天苷(SAL)对小鼠成牙本质细胞系MDPC⁃23增殖、迁移和矿化能力的影响。方法:体外培养小鼠成牙本质细胞系MDPC⁃23,用不同浓度SAL(0、10、20、50、100μmol/L)培养MDPC⁃23,通过CCK⁃8法、活/死细胞染色、划痕实验、Transwell实验检验SAL对MDPC⁃23毒性、增殖和迁移能力影响。对0、10、20μmol/L SAL组MDPC⁃23进行矿化诱导培养,通过碱性磷酸酶(ALP)染色及活性检测、茜素红染色、钙结节定量测定、实时荧光定量PCR和Western blot实验评估各组细胞矿化能力。结果:SAL可促进细胞的增殖且无明显细胞毒性,以20μmol/L效果最为显著(P<0.05);SAL对细胞迁移有显著促进作用(P<0.05)。SAL诱导MDPC⁃23产生更多的矿化结节(P<0.05)。20μmol/L SAL显著促进ALP表达(P<0.05)。SAL处理MDPC⁃23后的ALP、骨钙素(OCN)和Runt相关转录因子2(Runx2)、牙本质涎磷蛋白(DSPP)以及牙本质基质蛋白⁃1(DMP⁃1)的基因表达显著增加(P<0.05),OCN和Runx2蛋白表达水平显著增加(P<0.05)。结论:SAL可促进MDPC⁃23的增殖、迁移和矿化。Objective:To investigate the influence of salidroside(SAL)on the proliferation,migration and mineralization abilities of the mouse odontoblast cell line MDPC⁃23.Methods:MDPC⁃23 cells were cultured in vitro and stimulated with different concentra⁃tions of salidroside(0μmol/L,10μmol/L,20μmol/L,50μmol/L and 100μmol/L).The effects of salidroside on MDPC⁃23 prolif⁃eration and migration were examined by using CCK⁃8,live⁃dead cell staining,scratch assay and Transwell assay.Three groups were set up:control group(osteogenic medium),and 10μmol/L SAL group and 20μmol/L SAL group.The mineralization capacity of SAL was evaluated by alkaline phosphatase(ALP)staining,ALP activity assay,alizarin red staining(ARS),calcium nodule quantifica⁃tion,qRT⁃PCR and western blot.Results:SAL promoted cell proliferation without significant cytotoxicity,with the most significant effect at 20μmol/L(P<0.05).SAL promoted cell proliferation across the experimental concentration range,with 20μmol/L SAL ex⁃hibiting a significant proliferative effect.SAL had no significant cytotoxicity.SAL significantly enhanced cell migration(P<0.05).SAL treatment resulted in a greater number of mineralized nodules(P<0.05).ALP staining and activity assays indicated that 20μmol/L SAL significantly promoted ALP expression(P<0.05).The expression of ALP,osteocalcin(OCN),Runt⁃related transcription factor 2(Runx2),dentin sialophosphoprotein(DSPP),and dentin matrix protein⁃1(DMP⁃1)were significantly increased(P<0.05),and the protein expression levels of OCN and Runx2 were also significantly increased after SAL treatment(P<0.05).Conclusions:SAL can promote the proliferation,migration and mineralization abilities of odontoblast cell line MDPC⁃23 in mice.

关 键 词：红景天苷 小鼠成牙本质细胞 盖髓剂 

分 类 号：R780.2[医药卫生—口腔医学]