二氧化钛纳米管涂层在去离子水中的自封闭效应对细胞的影响  

Effect of self-sealing effect of titanium dioxide nanotube coating on cells in deionized water

作　　者：李东雨 赵继荣 王明浩 朱小苗 赵领洲 何文喜 LI Dongyu;ZHAO Jirong;WANG Minghao;ZHU Xiaomiao;ZHAO Lingzhou;HE Wenxi(School of Life Sciences,Yan'an University,Yan'an 716000,China;State Key Laboratory of Military Stomatology,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Department of Endodontics,School of Stomatology,Air Force Medical University,Xi'an 710032,China;Department of Stomatology,Air Force Medical Center,Beijing 100142,China)

机构地区：[1]延安大学生命科学学院,陕西延安716000 [2]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学口腔医院牙体牙髓病科,陕西西安710032 [3]空军特色医学中心口腔科,北京100142

出　　处：《空军军医大学学报》2024年第10期1099-1103,共5页Journal of Air Force Medical University

基　　金：国家自然科学基金(81970932);陕西省自然科学基础研究计划项目(2022JZ-042)。

摘　　要：目的研究二氧化钛纳米管涂层在去离子水中的自封闭效应及其对大鼠骨髓间充质干细胞(BMSCs)和小鼠单核巨噬细胞(RAW264.7)增殖黏附的影响。方法利用阳极氧化法在空白组纯钛表面制备纳米管。空白钛片为对照组,将去离子水处理二氧化钛纳米管0、1、3、5、7 d为实验组,观察材料表征,并在6组材料表面分别孵育BMSCs和RAW264.7,使用扫描电镜观察种植体表面涂层及细胞形态。结果通过观察去离子水浸泡研究二氧化钛纳米管涂层阳极氧化膜在去离子水中的自封闭效应,发现先在管壁形成针形颗粒样结构;随着浸泡时间的延长,3 d后更多的颗粒覆盖于纳米管口上;5 d左右形成了“花环”样结构;7 d针刺样结构完全封闭下方的纳米管口。结果表明,阳极氧化薄膜在去离子水中浸泡初期存在一个明显的自封闭过程,结合扫描电镜宏观观察到氧化膜多孔层的变化。结论去离子水处理纳米管会影响二氧化钛纳米管管壁产生新的自封闭结构。去离子水环境下二氧化钛纳米管相对抑制BMSCs和RAW264.7的增殖及早期铺展。Objective To investigate the self-sealing effect of titanium dioxide nanotube coating in deionized water and its effect on the proliferation and adhesion of rat bone marrow mesenchymal stem cells(BMSCs)and mouse mononuclear macrophages(RAW264.7).Methods Nanotubes were prepared on the surface of pure titanium in blank group by anodic oxidation method.Blank titanium sheet was used as the control group,and deionized water-treated titanium dioxide nanotubes for 0,1,3,5 and 7 d were used as the experimental group.The material characterization was observed,and BMSCs and RAW264.7 were incubated on the surfaces of the six groups,respectively.The surface coating and cell morphology of the implants were observed by scanning electron microscopy.Results The self-sealing effect of titanium dioxide nanotube coated anodic oxide film in deionized water was studied by observing the immersion in deionized water.With the extension of soaking time,more particles covered the nanotubes after 3 d.After 5 d,a“garland”-like structure was formed.The needle-like structure completely closed the nanotubes below after 7 d.The results showed that there was an obvious self-sealing process in the initial stage of the anodized film soaking in deionized water,and the changes of porous layer of the anodized film were observed by scanning electron microscopy.Conclusion Deionized water-treated titania nanotubes can affect the formation of new self-sealing structures on the wall of titania nanotubes.The proliferation and early spread of BMSCs and RAW264.7 are inhibited by titania nanotubes in deionized water.

关 键 词：二氧化钛纳米管 种植体 去离子水 自封闭效应 改性 

分 类 号：R783[医药卫生—口腔医学]