Epidermal microorganisms contributed to the toxic mechanism of nZVI and TCEP in earthworms by robbing metal elements and nutrients  

作　　者：Jie Hou Meirui Yang Xinyue Wu Qiqi Chen Yuqi Lu Jianying Zhang Daohui Lin 

机构地区：[1]Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control,Department of Environmental Science,Zhejiang University,Hangzhou 310058,China [2]Zhejiang Ecological Civilization Academy,Anji 313300,China [3]National Demonstration Center for Experimental Environment and Resources Education(Zhejiang University),Hangzhou 310058,China

出　　处：《Eco-Environment & Health》2024年第1期80-88,共9页生态环境与健康（英文）

基　　金：supported by the National Key Research and Development Program of China(2022YFC3702103);the Natural Science Foundation of China(U21A20163,42192573,22376181);the Zhejiang Provincial Natural Science Foundation of China(LD21B070001).

摘　　要：Disrupting effects of pollutants on symbiotic microbiota have been regarded as an important mechanism of host toxicity,with most current research focusing on the intestinal microbiota.In fact,the epidermal microbiota,which participates in the nutrient exchange between hosts and environments,could play a crucial role in host toxicity via community changes.To compare the contributions of intestinal and epidermal symbiotic microorganisms to host toxicity,this study designed single and combined scenarios of soil contamination[nano zerovalent iron(nZVI)and tris(2-chloroethyl)phosphate(TCEP)],and revealed the coupling mechanisms between intestinal/epidermal symbiotic bacterial communities and earthworm toxicological endpoints.Microbiome analysis showed that 15%of intestinal microbes were highly correlated with host endpoints,compared to 45%of epidermal microbes showing a similar correlation.Functional comparisons revealed that key species on the epidermis were mainly heterotrophic microbes with genetic abilities to utilize metal elements and carbohydrate nutrients.Further verifications demonstrated that when facing the co-contamination of nZVI and TCEP,certain symbiotic microorganisms became dominant and consumed zinc,copper,and manganese along with saccharides and amino acids,which may be responsible for the nutritional deficiencies in the host earthworms.The findings can enrich the understanding of the coupling relationship between symbiotic microorganisms and host toxicity,highlighting the importance of epidermal microorganisms in host resistance to environmental pollution.

关 键 词：Microbial community Host Soil pollution Joint toxicity Heterotrophic function 

分 类 号：X172[环境科学与工程—环境科学]