高效液相色谱-示差折光检测器法测定牛磺酸颗粒中牛磺酸  

Determination of taurine in Taurine Granules by HPLC-RID

作　　者：覃婷婷 房思萌 王培 鲁鑫 白海娇 韩晓捷 QIN Tingting;FANG Simeng;WANG Pei;LU Xin;BAI Haijiao;HAN Xiaojie(Tianjin Institute of Drug Control,Tianjin 300070,China)

机构地区：[1]天津市药品检验研究院,天津300070

出　　处：《现代药物与临床》2024年第9期2276-2279,共4页Drugs & Clinic

基　　金：国家药品标准制修订研究课题(2021H032)。

摘　　要：目的 建立高效液相色谱-示差折光检测器(HPLC-RID)法测定牛磺酸颗粒中牛磺酸。方法 采用Welch Ultimate HILIC Amide色谱柱(250 mm×4.6 mm,5μm),以0.1%冰醋酸-乙腈(30∶70)为流动相,柱温为30℃,示差折光检测器温度30℃,体积流量1.0 mL/min,进样体积20μL。结果 牛磺酸在0.008~1.211 mg/mL与峰面积呈良好线性关系,平均回收率为99.9%,RSD值为0.7%。结论 本法测定准确、可靠、专属性强,为牛磺酸制剂的测定提供新的检测方法。Objective To establish an HPLC-RID method for determination of taurine in Taurine Granules.Methods Welch Ultimate HILIC Amide column(250 mm×4.6 mm,5μm)was used.The mobile phase was 0.1%glacial acetic acid-acetonitrile(30:70).The column temperature was 30 C,and the'differential refractive detector temperature was 30 C.The volume flow rate was 1.0 mL/min,and the injection volume was 20μL.Results Taurine had good linear relationships of 0.008—1.211 mg/mL,and the average recovery rate was 99.9%with RSD 0.7%.Conclusion This method is accurate,reliable,and highly specific,which provides a new detection method for the determination of taurine preparations.

关 键 词：牛磺酸颗粒 牛磺酸 HPLC-RID 

分 类 号：R286.02[医药卫生—中药学]