组培容器清脂处理装置研发与试验  

Development and testing of a device for cleaning agar in tissue culture containers

作　　者：郑航 林文英 张毅 薛向磊 叶云翔 陈兵[1,2] 俞国红 ZHENG Hang;LIN Wenying;ZHANG Yi;XUE Xianglei;YE Yunxiang;CHEN Bing;YU Guohong(Institute of Agricultural Equipment,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang;Key Laboratory of Agricultural Equipment for Hilly and Mountainous Areas in Southeastern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Hangzhou 310021,Zhejiang;Songyang County Agriculture and Rural Bureau,Songyang 323400,Zhejiang;Zhejiang Qiantang Robot&Intelligent Equipment Research Co.,Ltd.,Hangzhou 310021,Zhejiang)

机构地区：[1]浙江省农业科学院农业装备研究所,浙江杭州310021 [2]农业农村部东南丘陵山地农业装备重点实验室(部省共建),浙江杭州310021 [3]松阳县农业农村局,浙江松阳323400 [4]浙江钱塘机器人及智能装备研究有限公司,浙江杭州310021

出　　处：《浙江农业科学》2024年第10期2338-2343,共6页Journal of Zhejiang Agricultural Sciences

基　　金：浙江省“三农九方”科技协作项目(2022R21S61D01)。

摘　　要：针对现有组培容器因琼脂黏杯壁导致自动清洗难的问题,提出了一种机械式离心技术清理组培琼脂的方法。通过单因素试验,探究了清脂刮片转速、刮片旋转时间、刮片尺寸以及刮片进出瓶口方式对容器清脂效果的影响。采用正交试验得到了最优试验条件组合,结果表明,刮片旋转速度210 r·min^(-1)、刮片旋转时间2.5 s、刮片进出方式为正转-反转、刮片半径为28 mm,组培容器清脂效果达到95%以上,能满足组培容器清洗灌装实际生产需求。该试验结果为回收型组培容器自动清洗灌装装备研发提供技术支持,具备工程化推广意义。A mechanical centrifugal technique was proposed to clean the agar in the tissue culture containers because of the difficulty of automatic cleaning due to the agar sticking to the wall of the cups.The effects of the rotation speed of the scraper,the rotation time of the scraper,the size of the scraper and the way of scraping in and out of the bottle on the cleaning effect of the containers by a one-way test.The orthogonal test was used to obtain the optimal combination of test conditions,and the results showed that the scraper rotation speed was 210 r·min^(-1),the scraper rotation time was 2.5 s,the scraper in/out mode was positive-reverse rotation,the scraper radius was 28 mm,the effect of agar clearing of the container in the tissue culture reached more than 95%,which can meet the actual production requirements of the tissue culture container cleaning and filling.The test results provide technical support for the research and development of automatic cleaning and filling equipment for recycling-type tissue culture containers,and have the significance of engineering promotion.

关 键 词：组培 琼脂 清脂试验 清脂效果 

分 类 号：S226.5[农业科学—农业机械化工程]