Plant-microbial interactions inspired the discovery of novel sesquiterpenoid dimeric skeletons of hidden natural products from Hibiscus tiliaceus  

作　　者：Deli Chen Jiawen Li Xudong Xu Zhaocui Sun Yun Yang Minghui Xu Hanqiao Liang Junshan Yang Hui Meng Guoxu Ma Jianhe Wei 

机构地区：[1]Hainan Branch of the Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Medical Sciences&Peking Union Medical College,Haikou 570311,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China [3]School of Pharmaceutical Science,Hainan Medical University,Haikou 571199,China [4]Department of Biomedicine,Beijing City University,Beijing 100094,China

出　　处：《Chinese Chemical Letters》2024年第10期286-290,共5页中国化学快报（英文版）

基　　金：financially supported by the CAMS Innovation Fund for Medical Sciences(CIFMS)(Nos.2021-I2M-1-032,2021I2M-1-071);the Key Research Project of Hainan Province(No.ZDYF2020111);Project supported by the Research Fund for Development Program of Beijing City University(No.KYF202003)。

摘　　要：Hidden natural products are representative of defensive strategies produced in vivo in diseased plants,a process that is induced by the plant immune system.The first transcriptome library of uninfected and pathogen infected Hibiscus tiliaceus stems was constructed by transcriptome sequencing technology,genes related to cadinene-type sesquiterpenoid biosynthesis were screened and combined with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-QTOF-MS)analysis data,which indicated pathological tissue had potential to produce novel carbon skeletons of cadinane sesquiterpenoid dimers.Successfully,two cadinane-derived sesquiterpenoid dimers with unprecedented carbon skeletons,hibisceusanols A(1)and B(2)were isolated for the first time from the stems of H.tiliaceus induced by plant-microbial interactions.Their structures and absolute configurations were unambiguously established by spectroscopy,advanced chemistry development(ACD)and electronic circular dichroism(ECD)methods.Compounds 1 and 2 exhibited significant antitumor activity in vitro with half maximal inhibitory concentration(IC_(5)0)values of 2.3–7.2μmol/L.The anticancer effect was generated via the induction of Hep G2 cell apoptosis by inhibiting the phosphatidylinositol 3-kinase(PI3K)pathway.

关 键 词：Cadinane-derived sesquiterpenoid ANTI-CANCER PI3K Plant-microbial interactions 

分 类 号：O629[理学—有机化学] TQ460.1[理学—化学]