WISP-1与VEGF-A在老年食管鳞癌组织中表达的临床意义  

Clinical Significance of WISP-1 and VEGF-A Expression Levels in Elderly Patients with Esophageal Squamous Cell Carcinoma

作　　者：李晨露 黄俊星 LI Chenlu;HUANG Junxing(Department of Oncology,The Affiliated Taizhou People’s Hospital of Nanjing Medical University,Taizhou 225300,China)

机构地区：[1]南京医科大学附属泰州人民医院肿瘤科,泰州225300

出　　处：《肿瘤防治研究》2024年第10期858-863,共6页Cancer Research on Prevention and Treatment

基　　金：江苏省卫生健康委科研项目(BJ20012)。

摘　　要：目的探索WNT1诱导信号通路蛋白-1(WISP-1)及血管内皮生长因子-A(VEGF-A)在老年食管鳞状细胞癌(ESCC)患者中作为新的分子标志物的临床价值。方法将老年ESCC分为65~70岁组与>70岁组,分别应用免疫组织化学染色法与RT-PCR检测WISP-1、VEGF-A蛋白与m RNA的表达。Log rank检验分析临床病理特征与食管鳞癌预后的关系。结果ESCC组织中WISP-1阳性表达率明显高于癌旁组织(53.33%vs.35.42%,P=0.045)、>70岁及有淋巴结转移的患者显著高于65~70岁(P=0.047)及无淋巴结转移的患者(P=0.032)。ESCC组织中VEGF-A蛋白的阳性表达率显著高于癌旁组织(50.00%vs.20.83%,P=0.001),浸润程度T3~T4期、有淋巴结转移、TNM分期Ⅲ~Ⅳ期显著高于T1~T2、无淋巴结转移、Ⅰ~Ⅱ期患者(P=0.030、0.006、0.010)。WISP-1mRNA和VEGF-A mRNA表达水平明显高于癌旁组织(P<0.05)。单因素分析结果表明WISP-1及VEGF-A表达水平与老年ESCC的预后相关(P<0.05),多因素分析结果表明淋巴结转移是影响老年ESCC患者预后的独立危险因素。结论WISP-1及VEGF-A与老年ESCC患者恶性行为相关,且年龄越大,WISP-1表达越高,有作为老年ESCC新的分子标志物的潜能。Objective To investigate the clinical value of WNT1 inducible signaling pathway protein 1(WISP-1)and vascular endothelial growth factor-A(VEGF-A)as novel molecular markers of esophageal squamous cell carcinoma(ESCC)in elderly patients.Methods Elderly patients with ESCC were divided into 65-70 years old group and>70 years old group.Immunohistochemical staining and RT-PCR were used to detect the expression levels of WISP-1 and VEGF-A proteins and mRNAs,respectively.Log rank test was used to analyze correlation between clinicopathological features and prognosis of ESCC in the elderly.Results The positive expression rate of WISP-1 in ESCC tissues was significantly higher than that in paracancer tissues(53.33%vs.35.42%,P=0.045).The positive expression rate of WISP-1 in patients over 70 years old and with lymph node metastasis was significantly higher than that in patients aged 65-70 years and without lymph node metastasis(P=0.047 and 0.032).The positive expression rate of VEGF-A protein in ESCC tissues was significantly higher than that in para-cancer tissues(50.00%vs.20.83%,P=0.001).The positive expression rate of VEGF-A in patients with depth of infiltration T3-T4 stage,lymph node metastasis,and TNMⅢ-Ⅳstage was significantly higher than that in patients with T1-T2 stage,no lymph node metastasis,andⅠ-Ⅱstage(P=0.030,0.006,and 0.010,respectively).The mRNA expression levels of these two molecules were significantly higher in ESCC tissues than in para-cancer tissues(P<0.05),and the results of univariate analysis showed that the expression levels of WISP-1 and VEGF-A were correlated with the prognosis of ESCC in the elderly(P<0.05).The results of multivariate analysis showed that lymph node metastasis was the independent risk factor affecting the prognosis of elderly patients with ESCC.Conclusion WISP-1 and VEGF-A are associated with malignant behavior in elderly patients with ESCC.The expression of WISP-1 increased with age,and it has potential as a novel molecular marker of ESCC in elderly patients.

关 键 词：老年 食管鳞癌 WISP-1 VEGF-A 

分 类 号：R735.1[医药卫生—肿瘤]