结直肠癌组织中lncRNA CCAT2和NDRG1的表达及意义  

Expression and significance of lncRNA CCAT2 and NDRG1 in colorectal cancer tissues

作　　者：周钰杰[1] 杨芳[2] 严晶[3] 钱政[1] ZHOU Yujie;YANG Fang;YAN Jing;QIAN Zheng(Department of Proctology,Nantong Hospital of Traditional Chinese Medicine,Nantong,Jiangsu 226001,China;Department of Gastroenterology,Nantong Hospital of Traditional Chinese Medicine,Nantong,Jiangsu 226001,China;the First Clinical Medical College of Nanjing University of Chinese Medicine,Nantong,Jiangsu 210023,China)

机构地区：[1]南通市中医院肛肠科,江苏南通226001 [2]南通市中医院消化内科,江苏南通226001 [3]南京中医药大学第一临床医学院,江苏南通210023

出　　处：《国际检验医学杂志》2024年第20期2437-2442,共6页International Journal of Laboratory Medicine

基　　金：国家自然科学基金项目(81503536)。

摘　　要：目的探讨结直肠癌(CRC)患者组织中长链非编码RNA(lncRNA)结肠癌相关转录物2(CCAT2)、N-myc下游调节基因(NDRG)1的表达及与临床病理特征及预后的关系。方法选取2018年2月至2020年2月在南通市中医院行CRC根治性手术治疗的96例CRC患者作为研究对象。采用实时荧光定量PCR检测组织中lncRNA CCAT2、NDRG1 mRNA表达,采用免疫组织化学检测组织中NDRG1蛋白表达,采用Kaplan-Meier曲线分析不同lncRNA CCAT2、NDRG1 mRNA表达组患者的预后差异,采用多因素Cox回归分析CRC预后影响因素。结果与癌旁组织比较,癌组织中lncRNA CCAT2表达较高,NDRG1 mRNA表达及蛋白阳性率较低,差异有统计学意义(P<0.05)。与TNM分期Ⅰ~Ⅱ期、无淋巴结转移比较,TNM分期Ⅲ期、有淋巴结转移的CRC患者癌组织中lncRNA CCAT2表达较高,NDRG1 mRNA表达较低(P<0.05)。lncRNA CCAT2高表达组3年累积生存率低于lncRNA CCAT2低表达组,而NDRG1 mRNA高表达组3年累积生存率高于NDRG1 mRNA低表达组,差异有统计学意义(P<0.05)。TNM分期、淋巴结转移,lncRNA CCAT2、NDRG1 mRNA是CRC预后影响因素(P<0.05)。结论CRC组织中lncRNA CCAT2表达升高,NDRG1表达降低,二者均参与CRC肿瘤的进展,可作为评估CRC患者生存预后的新指标。Objective To investigate the expression of long non-coding RNA(lncRNA)colon cancer associated transcript 2(CCAT2)and N-myc downstream regulated gene(NDRG)1 in colorectal cancer(CRC)tissues and their relationship with clinicopathological features and prognosis.Methods A total of 96 patients with CRC who underwent radical surgery in Nantong Hospital of Traditional Chinese Medicine from February 2018 to February 2020 were selected as the research objects.Quantitative real-time PCR was used to detect the mRNA expression of lncRNA CCAT2 and NDRG1 in tissues.The expression of NDRG1 protein was detected by immunohistochemistry.Kaplan-Meier curve was used to analyze the prognosis differences of patients with different lncRNA CCAT2 and NDRG1 mRNA expression groups.Multivariate Cox regression was used to analyze the prognostic factors of CRC.Results The expression of lncRNA CCAT2 in cancer tissues was higher than that in adjacent tissues,and the expression of NDRG1 mRNA and protein was lower than that in adjacent tissues(P<0.05).The expression of lncRNA CCAT2 was higher and the expression of NDRG1 mRNA was lower in TNM stageⅢCRC patients with lymph node metastasis than in TNM stageⅠ—ⅡCRC patients without lymph node metastasis(P<0.05).The 3-year cumulative survival rate of lncRNA CCAT2 high expression group was lower than that of lncRNA CCAT2 low expression group,while the 3-year cumulative survival rate of NDRG1 mRNA high expression group was higher than that of NDRG1 mRNA low expression group,and the difference was statistically significant(P<0.05).TNM stage,lymph node metastasis,lncRNA CCAT2 and NDRG1 mRNA were the prognostic factors of CRC(P<0.05).Conclusion The expression of lncRNA CCAT2 is increased and the expression of NDRG1 is decreased in CRC tissues.Both lncrna CCAT2 and NDRG1 are involved in the progression of CRC and can be used as new indicators for evaluating the survival and prognosis of CRC patients.

关 键 词：结直肠癌 长链非编码RNA结肠癌相关转录物2 N-myc下游调节基因1 预后 

分 类 号：R735.34[医药卫生—肿瘤]