超声波辅助酶法提取猪皮胶原蛋白的工艺优化及胶原纤维的湿法纺丝制备  

Process optimization of ultrasonic-assisted enzymatic extraction of pigskin collagen and preparation of collagen fiber by wet spinning

作　　者：徐敏杰 唐树淇 胡敏 蔡光明 张如全[1,2] 岳程飞 XU Minjie;TANG Shuqi;HU Min;CAI Guangming;ZHANG Ruquan;YUE Chengfei(School of Textile and Engineering,Wuhan Textile University,Wuhan 430200,China;State Key Laboratory of New Textile Materials and Advanced Processing Technologies,Wuhan Textile University,Wuhan 430200,China)

机构地区：[1]武汉纺织大学纺织科学与工程学院,武汉430200 [2]武汉纺织大学省部共建纺织新材料与先进加工技术国家重点实验室,武汉430200

出　　处：《纺织工程学报》2024年第5期40-50,共11页JOURNAL OF ADVANCED TEXTILE ENGINEERING

基　　金：武汉市科技局应用基础前沿项目(2022013988065196)。

摘　　要：胶原纤维材料是一种重要的生物医用纺织品,广泛用于各类外科手术。为了提高猪皮源胶原蛋白的提取率,设定酶浓度、超声强度、超声时间为影响因素,胶原提取率为响应值,采用超声波辅助酶法,通过单因素实验和Box-Behnken响应面法探究最佳提取条件,并采用湿法纺丝技术制备猪皮源胶原纤维。结果表明:影响胶原提取率的显著性顺序为:酶浓度>超声时间>超声强度,最佳提取工艺的酶浓度为93 U/g、超声强度为162 W、超声时间为58 min,所制备的胶原提取率56.21%,与响应面回归方程预测值相近,说明该回归模型能够较好地优化提取工艺条件。同时,紫外光谱、红外光谱和X射线衍射分析表明猪皮源胶原的类型为Ⅰ型,具有完整的三螺旋结构;纤维形貌分析表明猪皮源胶原具有良好的成纤性;力学性能分析表明纺丝液为4.0%浓度的胶原纤维具有最佳的力学性能。Collagen fiber material is an important biomedical textile widely used in various types of surgical procedures.In order to improve the extraction rate of pigskin-derived collagen,the enzyme concentration,ultrasound intensity,and ultrasound time were set as the influencing factors,and the collagen extraction rate was set as the response value,and the ultrasound-assisted enzyme method was used to explore the optimal extraction conditions through one-way experiments and the Box-Behnken response surface method,and the wet spinning technique was used to prepare pigskin-derived collagen fibers.The results showed that the order of significance affecting the collagen extraction rate was:enzyme concentration>ultrasound time>ultrasound intensity,and the optimal extraction process had an enzyme concentration of 93 U/g,an ultrasound intensity of 162 W,and an ultrasound time of 58 min,and the collagen extraction rate of the prepared collagen was 56.21%,which was similar to that predicted by response surface regression equations,suggesting that the regression model could optimize the conditions of the extraction process better.Meanwhile,ultraviolet spectroscopy,infrared spectroscopy and X-ray diffraction analyses showed that the type of pigskin-derived collagen was type Ⅰ,with a complete triple helix structure;fiber morphology analysis showed that pigskin-derived collagen had good fiber-forming properties;and mechanical property analysis showed that collagen fibers with a spinning solution of 4.0%concentration had the best mechanical properties.

关 键 词：猪皮胶原蛋白 超声波辅助酶法 响应面法 胶原纤维 湿法纺丝 

分 类 号：TS102.5[轻工技术与工程—纺织工程]