非肥胖型与肥胖型非酒精性脂肪肝血清脂质组学研究  

Serum lipidomics analysis in non-obese and obese patients with non-alcoholic fatty liver disease

作　　者：丁荣蓉[1] 周新兰[1] 李秀芬[1] 黄丹[1] 林维佳 陈良[1] DING Rong-rong;ZHOU Xin-lan;LI Xiu-fen;HUANG Dan;LIN Wei-jia;CHEN Liang(Department of Hepatobiliary Medicine,Shanghai Public Health Clinical Center,Shanghai 201508,China)

机构地区：[1]上海市公共卫生临床中心肝胆内科,201508

出　　处：《肝脏》2024年第8期975-979,共5页Chinese Hepatology

基　　金：上海市金山区医药卫生类科技创新资金项目(2021-3-55)。

摘　　要：目的比较非肥胖型与肥胖型非酒精性脂肪性肝病(NAFLD)血清脂质谱的差异。方法纳入2023年1月至12月上海市公共卫生临床中心诊治的NAFLD患者60例,根据BMI分为非肥胖型组(BMI<25 kg/m^(2))和肥胖型组(BMI≥25 kg/m^(2))。超高效液相色谱-质谱检测非肥胖型NAFLD和肥胖型NAFLD患者的血清脂质代谢物。采用差异倍数、偏最小二乘判别(PLS-DA)和正交偏最小二乘法-判别分析(OPLS-DA)并进行置换检验,筛选差异脂质。结果共筛选出90种差异脂质。包括鞘磷脂(SM)、醚甘油磷脂酰胆碱(EtherPC)、神经酰胺(HexCer)、磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)等。KEGG通路富集分析显示非肥胖型NAFLD患者的一些代谢途径发生明显变化,包括鞘脂信号通路、坏死性凋亡、甘油磷脂代谢通路、糖基磷脂酰肌醇的合成途径、肿瘤的胆碱代谢通路等。结论非肥胖型NAFLD和肥胖型NAFLD的血清脂质谱存在一定差异,包括PC、PE、SM等。Objective To compare the serum lipid profiles between non obese and obese patients with non-alcoholic fatty liver disease(NAFLD)patients.Methods A total of 60 NAFLD patients were recruited and categorized into two groups based on body mass index(BMI):non-obese(BMI<25 kg/m^(2))and obese(BMI≥25 kg/m^(2)).Serum samples from all patients were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS)for lipidomics profiling.Differential lipids were identified through multiple statistical methods,including fold change anaysis,partial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA).Permutation tests were conducted to validate the results.Results A total of 90 differential lipids were identified,Including sphingomyelin(SM),Ether linked phosphatidylcholine(EtherPC),hexosylceramides(HexCer),and phosphatidylethanolamine(PC).KEGG pathway enrichment analysis revealed significant alterations in several metabolic pathways in non-obese NAFLD patients,such as the sphingolipid signaling pathway,necroptosis,glycerophospholipid metabolism pathway,glycosylphosphatidylinositol biosynthetic pathway,and tumor choline metabolism pathway.Conclusion Significant differences in serum lipid mass spectrometry profiles were observed between non-obese and obese NAFLD patients,particularly in PC,PE,SM levels.

关 键 词：非酒精性脂肪性肝病 脂质组学 肥胖 

分 类 号：R575.5[医药卫生—消化系统]