东南景天内生菌对小麦锌吸收转运的影响  

Effects of endophytes from Sedum alfredii on zinc uptake and transport in wheat

作　　者：李喆思 廖佳源 刘婵娟 黄路宽 冯英[1] LI Zhesi;LIAO Jiayuan;LIU Chanjuan;HUANG Lukuan;FENG Ying(Key Laboratory of Environment Remediation and Ecological Health,Ministry of Education,College of Environmental Resource Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学环境与资源学院,污染环境修复与生态健康教育部重点实验室,杭州310058

出　　处：《农业环境科学学报》2024年第10期2229-2239,共11页Journal of Agro-Environment Science

基　　金：国家自然科学基金面上项目(42277002);浙江省自然科学基金重点项目(LZ22D010002)。

摘　　要：为探究超积累植物特异内生菌对非宿主作物锌(Zn)营养的调控作用,采用水稻土(Paddy soil,PS)和潮土(Fluvo-aquic soil,FAS)开展盆栽试验,设置10种不同内生细菌灌根处理,以探究内生菌对小麦幼苗Zn吸收转运的影响。结果表明:在Zn含量较为充足的PS中,接种SaPS1使地上部生物量增加了158.48%,接种SaPA1使地上部Zn含量提高了108.83%,而在Zn含量较为缺乏的FAS中,接种SaVA1使地上部生物量增加了60.16%,接种SaZR8和SaBR2使根部Zn含量提高了74.17%和73.02%。实时荧光定量分析结果显示,在PS中地上部TaZIP3、TaZIP6、TaZIP7和TaZIP10的表达水平在接种SaPA1和SaPS1后提高超过100%,而在FAS中,接种SaBR2后地上部和根部这4个基因的表达量均极显著增加。根际微生物群落16S rDNA测序结果发现,虽然SaPA1和SaPS1灌根处理对根际微生物群落多样性无显著影响,但改变了部分功能微生物的丰度。这些研究结果表明,在Zn含量不同的土壤中,东南景天特异内生菌可以调节小麦对Zn的吸收转运,可作为新型生物制剂用于小麦Zn营养生物强化。In order to investigate the regulation mechanism of specific endophytes from hyperaccumulator plants on non-host crops zinc(Zn)nutrition,a pot experiment was carried out by using paddy soil and fluvo-aquic soil(FAS),and 10 different endophytic bacteria treatments were set up to investigate the effect of endophytes on Zn uptake and transport in wheat.The results showed that in Zn-sufficient paddy soil,inoculation with SaPS1 increased shoot biomass by 158.48%,and inoculation with SaPA1 increased shoot Zn concentration by 108.83%.In Zn-deficient fluvo-aquic soil,inoculation with SaVA1 increased shoot biomass by 60.16%,and inoculation with SaZR8 and SaBR2 increased root Zn concentration by 74.17%and 73.02%.Real-time PCR showed that in paddy soil,the expression of TaZIP3,TaZIP6,TaZIP7,and TaZIP10 in shoots increased by more than 100%after inoculation with SaPA1 and SaPS1.In FAS,the expression of these four genes in both shoots and roots significantly increased after inoculation with SaBR2.Rhizosphere microbial communities sequencing of 16S rDNA revealed that although inoculation with SaPA1 and SaPS1 did not significantly affect the diversity of rhizosphere microbial communities,it altered the abundance of some functional microorganisms.These results suggest that specific endophytes from Sedum alfredii regulate Zn uptake and transport in wheat under different soil Zn conditions and are potential candidates for new Zn biofortification fertilizers.

关 键 词：超积累植物 共生微生物 菌剂 Zn 生物强化 

分 类 号：X17[环境科学与工程—环境科学]