Titanium Culture Vessel Presenting Temperature Gradation for the Thermotolerance Estimation of Cells  

作　　者：Chikahiro Imashiro Yangyan Jin Motoaki Hayama Takahiro GYamada Akira Funahashi Katsuhisa Sakaguchi Shinjiro Umezu Jun Komotori 

机构地区：[1]Graduate School of Engineering,The University of Tokyo,Tokyo 113-0033,Japan [2]Department of Mechanical Engineering,Keio University,Yokohama,Kanagawa 223-0061,Japan [3]School of Integrated Design Engineering,Graduate School of Science and Technology,Keio University,Yokohama,Kanagawa 223-0061,Japan [4]Department of Biosciences and Informatics,Keio University,Yokohama,Kanagawa 223-0061,Japan [5]Department of Integrative Bioscience and Biomedical Engineering,Graduate School of Advanced Science and Engineering,Waseda University,TWIns,Tokyo 162-8480,Japan [6]Department of Modern Mechanical Engineering,Waseda University,Tokyo 169-8555,Japan

出　　处：《Cyborg and Bionic Systems》2023年第1期166-177,共12页类生命系统（英文）

基　　金：supported by JSPS KAKENHI(Grant Nos.20J00337 and 22K18188);a grant from the Tateisi Science and Technology Foundation。

摘　　要：Hyperthermia can be induced to exploit the thermal intolerance of cancer cells,which is worse than that of normal cells,as a potential noninvasive cancer treatment.To develop an effective hyperthermia treatment,thermal cytotoxicity of cells should be comprehensively investigated.However,to conduct such investigations,the culture temperature must be accurately regulated.We previously reported a culture system in which the culture temperature could be accurately regulated by employing metallic culture vessels.However,appropriate temperature conditions for hyperthermia depend on the cell species.Consequently,several experiments need to be conducted,which is a bottleneck of inducing hyperthermia.Hence,we developed a cell culture system with temperature gradation on a metallic culture surface.Michigan Cancer Foundation-7 cells and normal human dermal fibroblasts were used as cancer and normal cell models,respectively.Normal cells showed stronger thermal tolerance;this was because the novel system immediately exhibited a temperature gradation.Thus,the developed culture system can be used to investigate the optimum thermal conditions for effective hyperthermia treatment.Furthermore,as the reactions of cultured cells can be effectively assessed with the present results,further research involving the thermal stimulation of cells is possible.

关 键 词：stimulation CULTURE TITANIUM 

分 类 号：R730[医药卫生—肿瘤]