Tumor Receptor-Seeking Dyes for Rapid Intraoperative Definition of Tumor Margin and Histopathological Morphology  

作　　者：Yajun Wang Feiran Zhang Yunlong Jia Tianyang Han Chengbin Zhang Limei Qu Yuewei Zhang Shoujun Zhu 

机构地区：[1]State Key Laboratory of Supramolecular Structure and Materials,Center for Supramolecular Chemical Biology,College of Chemistry,Jilin University,Changchun 130012 [2]Joint Laboratory of Opto-Functional Theranostics in Medicine and Chemistry,The First Hospital of Jilin University,Changchun 130021 [3]School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022 [4]Department of Pathology,The First Hospital of Jilin University,Changchun 130021

出　　处：《CCS Chemistry》2024年第10期2577-2593,共17页中国化学会会刊（英文）

基　　金：the National Key Research and Development Program of China(grant no.2022YFC2601900).

摘　　要：Histopathological diagnosis is a crucial part of surgical treatment,as it determines further treatment decisions and prognosis for patients.Due to the heterogeneity of tumors,traditional histopathological diagnosis relies on histological and cytological morphological analysis of cryo-slides by well-trained pathologists,which is subjective,time-consuming,and lacks considerable reliability.Therefore,we engineer a series of tumor receptor-seeking dyes to automatically label and visually depict the tumor contour with antibody-free interaction.The meso-chlorine on the cyclohexyl ring of dyes can covalently react with specific thiol groups in various tumor signature proteins to form stable dye@protein complexes.These dye@protein complexes have significantly amplified fluorescence signals in tumor sites,enabling highresolution delineation of tumormargins and providing histological morphology information to potentially determine tumor staging.The orthogonal effect not only avoids errors caused by individual signature proteins,but also amplifies the fluorescence difference between tumor and normal tissues.Notably,our method is easy to operate with a minimal detection time of only 4 min.The tumor receptor-seeking dyes successfully assess tumor-positive margins from 66 resected breast tumor samples and 25 intraoperative cancer patients with 100%accuracy,providing a newgeneration technique for intraoperative pathologic diagnosis of tumor biopsy.

关 键 词：tumor receptor-seeking dyes tumor-positive margins chemoselective covalent labeling nearinfrared imaging histopathological morphology 

分 类 号：R730.2[医药卫生—肿瘤]