野菊花的化学成分研究  

Chemical constituents of Chrysanthemum indicum

作　　者：任尧 韩依含 王奕航 胥鑫林 刘长鑫 谢治深 张振强[1] 王潘 REN Yao;HAN Yihan;WANG Yihang;XU Xinin;LIU Changxin;XIE Zhishen;ZHANG Zhenqiang;WANG Pan(Henan University of Chinese Medicine Collaborative Innovation Center of Research and Development on the Whole Industry Chain ofYuYao,Zhengzhou450046,China)

机构地区：[1]河南中医药大学豫药全产业链研发河南省协同创新中心,河南郑州450046

出　　处：《中草药》2024年第17期5770-5776,共7页Chinese Traditional and Herbal Drugs

基　　金：河南省高校科技创新人才(23HASTIT044,24HASTIT072);河南省科技攻关项目(232102310447);河南省自然科学基金联合基金(232301420085);中国博士后科学基金资助项目(2021M693704)。

摘　　要：目的研究野菊Chrysanthemum indicum花95%乙醇提取物的醋酸乙酯部位的化学成分。方法采用硅胶、Sephadex LH-20和半制备液相等色谱方法进行分离纯化,并运用现代波谱和质谱技术,以及化学方法鉴定所得化合物的结构,采用Nrf2-ARE荧光素酶报告基因系统评估化合物抗氧化活性。结果从野菊花中分离纯化得到12个化合物,分别鉴定为3-羟基-对异丙苄基-β-D-葡萄糖苷(1)、芝麻素(2)、(1S^(*),2R^(*),5S^(*),6S^(*))-2-(3,5-dimethoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(3)、槲皮素-3-O-β-D-葡萄糖苷(4)、圣草酚(5)、3',4',5,7-四羟基黄酮(6)、cirsilineol(7)、homoeriodictyol(8)、thymidine(9)、2E,4E,12E-tetra-decatriene-8,10-diynoic acid isobutylamide(10)、tetradeca-2E,4E,12Z-trien-8,10-diynoic acid isobutylamide(11)、α-亚麻酸(12)。结论其中化合物1为新化合物,命名为野菊苷;化合物3、12为菊科首分化合物。化合物7和11具有潜在的抗氧化活性。Objective To isolate and identify chemical constituents in the ethyl acetate part of 95%ethanol extracts of Chrysanthemum indicum.Methods Chromatographic methods such as silica gel,Sephadex LH-20 and semi preparative high performance liquid chromatography were used for the isolation and purification,and the structures of the obtained compounds were identified by nuclear magnetic resonance,mass spectrometry and other techniques.The Nrf2-ARE luciferase reporter gene system was used to evaluate the antioxidant activity of compounds.Results Twelve compounds were isolated and purified from the 95%ethanol extract of wild chrysanthemum,which were identified as 3-hydroxy-p-isopropylbenzyl-β-D-glucoside(1),sesamin(2),(1S^(*),2R^(*),5S^(*),6S^(*))-2-(3,5-dimethylphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane(3),quercetin-3-O-β-D-glucoside(4),eriodictyol(5),3',4',5,7-dioxabicyclo tetrahydroxyflavones(6),cirsilineol(7),homoeriodictyol(8),thymidine(9),2E,4E,12E-tetra-decatriene-8,10-diynoic acid isobutylamide(10),tetradeca-2E,4E,12Z-trien-8,10-diynoic acid isobutylamide(11),and alpha linolenic acid(12).Conclusion Compound 1 is a new compound named chrysanthoside,while compounds 3 and 12 are the first compounds in the Asteraceae family,and compounds 7 and 11 have potential antioxidant activity.

关 键 词：野菊花 糖苷 3-羟基-对异丙苄基-β-D-葡萄糖苷 野菊苷 cirsilineol Α-亚麻酸 

分 类 号：R284.1[医药卫生—中药学]