保存时间与温度对细胞程序性死亡配体1检测强度的影响  

Effect of both storage duration and temperature on programmed death-ligand 1 detection intensity

作　　者：杨敏 余蕾 孙情[1] 陈耿标[1] Yang Min;Yu Lei;Sun Qing;Chen Gengbiao(Department of Pathology,Sun Yat-sen Memorial Hospital of Sun Yat-sen University,Guangzhou 510120,China)

机构地区：[1]中山大学孙逸仙纪念医院病理科,广州510120

出　　处：《实用医技杂志》2024年第8期565-568,I0003,共5页Journal of Practical Medical Techniques

摘　　要：目的分析保存时间与温度对细胞程序性死亡配体1(PD-L1)检测强度的影响。方法将固定良好的正常扁桃体、胎盘组织和已知PD-L1阳性的口腔鳞癌组织制作成组织芯片,将组织芯片切涂胶白片36张,平均分成4组,每组9张白片,每张白片上均有3种组织;第1、2组不烤片,第3、4组于(58±2)℃烤箱烤片30 min;将第1、3组置于室温(25℃)冰箱保存,第2、4组置于-20℃冰箱保存。结果随着实验时间推移,在4组芯片的扁桃体隐窝上皮细胞、胎盘组织、口腔鳞癌的肿瘤细胞中,第1组、第3组芯片的阳性强度减弱较明显,第2组、第4组芯片的阳性强度减弱不明显,4组芯片两两比较可以得出,4组芯片在同等温度条件下的阳性强度均不受烤片与否的影响,-20℃冷冻保存的芯片相较于室温(25℃)保存的芯片阳性强度减弱不明显,抗原能获得更好的保存。结论PD-L1检测受保存时间与保存温度的影响。Objective To assess the impact of storage duration and temperature on the detection intensity of PD-L1(22C3).Methods Tissue microarrays were constructed using well-preserved samples of normal tonsil,placental tissue,and oral squamous cell carcinoma tissue,all known to exhibit PD-L1 positivity.A total of 36 gelatinized white tissue chips were excised and subsequently divided into four groups,with an average of nine white tissue samples per group.Each group contained a mix of the three tissue types.The first and second groups did not bake their slices,where as the third and fourth groups baked theirs in an oven at(58±2)℃for 30 minutes.Subsequently,the first and third groups stored their slices in a refrigerator set at room temperature(25℃),while the second and fourth groups placed theirs in a refrigerator at-20℃.Results Among the tonsil crypt epithelial cells,placental tissue,and tumor cells of oral squamous cell carcinoma in the four groups of chips,the positive intensity of the first and third groups of chips decreased significantly,while the positive intensity of the second and fourth groups of chips did not decrease significantly.Comparing the four groups of chips pairwise,it can be concluded that the positive intensity of the four groups of chips under the same temperature conditions remains unaffected regardless of whether they are baked or not.The positive intensity of chips stored at-20℃is not significantly reduced compared to those stored at room temperature(25℃),indicating better preservation of antigens.Conclusion The detection of PD-L1 is influenced by storage duration and temperature.

关 键 词：B7-H1抗原 程序性死亡配体1 免疫组织化学 抗原保存 

分 类 号：R361.2[医药卫生—病理学]