Phenolic profile and chronic gastric ulcer healing effects of Cameroonian propolis  

作　　者：Carol Elisabeth Domgnim Mokam Alfred Ngenge Tamfu Mimosette Mesmine Kuissu Selcuk Kucukaydin Christophe Mezui Enonchong George Enow-Orock Paul Vernyuy Tan 

机构地区：[1]Gastroenterology Research Unit,Animal Physiology Laboratory,Department of Animal Biology&Physiology,Faculty of Science,University of Yaoundé1,P.O.Box 812,Yaoundé,Cameroon [2]Department of Chemical Engineering,School of Chemical Engineering and Mineral Industries,University of Ngaoundere,P.O.Box 454,Ngaoundere,Cameroon [3]Department of Medical Services and Techniques,Koycegiz Vocational School of Health Services,Mugla Sitki Kocman University,P.O.Box 48800,Mugla,Turkey [4]Department of Animal Sciences,Faculty of Agriculture and Veterinary Medicine,University of Buea,P.O.Box 63,Buea,Cameroon [5]Department of Biological Sciences,Higher Teachers’Training College,University of Yaoundé1,P.O.Box 47,Yaoundé,Cameroon [6]Department of Biomedical Sciences,Faculty of Health Science,University of Buea,P.O.Box 63,Buea,Cameroon

出　　处：《Clinical Traditional Medicine and Pharmacology》2024年第3期35-45,共11页临床传统医学和药理学（英文）

摘　　要：Background:Gastric ulcers continue to affect millions of people worldwide,with reported cases of fatalities and the diagnosis,prevention and treatment constitute a burden on the patients especially in case of chronic ulcers which are difficult to treat.Natural medicines such as propolis provide suitable therapeutic alternatives in the treatment and curation of ulcers.Objective:This work focused on the evaluation of the phenolic profile and healing effect of Cameroonian propolis on gastric ulcers in rats.Methods:Identification and quantification of phenolic compounds in the propolis extract was done using HPLC-DAD.Chronic gastric ulcers were induced using ethanol(70%;1 mL/200 g p.o.)/indomethacin(1 mg/kg p.o.),and“unhealed”gastric ulcerations were induced using acetic acid(30%)/indomethacin(1 mg/kg p.o.).The animals were administered with the vehicle(Tween 20,1 mL/100 g p.o.),propolis extract(200 mg/kg,400 mg/kg and 600 mg/kg p.o.)or sucralfate(50 mg/kg p.o.),once daily for ten to fourteen days.At the end of each treatment period,the animals were sacrificed and ulcer indices,gastric mucus weight,histological and oxidative stress parameters were assessed.Results:Eight phenolic compounds were identified and quantified and most abundant were Caffeic acid(45.31±0.25μg/g),Chrysin(37.65±0.17μg/g)and p-Hydroxy benzoic acid(34.14±0.21μg/g).The treatment of ulcerated rats(chronic and unhealed gastric ulcer models)with propolis extract at 200 mg/kg,400 mg/kg and 600 mg/kg resulted in reduction of ulcer index compared to negative controls in both models.Histological ob-servation of stomach tissues showed that treatment with propolis extract led to a dose-dependent normalization of the mucosa.There was a significant increase(P<0.05;P<0.01 and P<0.001)in mucus weight that accom-panied the healing of the ulcers.Furthermore,doses of 200 mg/kg,400 mg/kg and 600 mg/kg of the propolis extract induced an increase in the amounts of SOD,catalase,nitrite and GSH while MDA concentrations decreased in both models.Conclusion:The

关 键 词：PROPOLIS HPLC-DAD Phenolics Anti-ulcer activity Gastric curative property In vivo antioxidant effects 

分 类 号：R573.1[医药卫生—消化系统]