母鼠妊娠期和哺乳期补充乳脂肪球膜对其子代骨骼生长和骨骼健康的影响  

Effects of Milk Fat Globule Membrane Supplementation to Rats During Pregnancy and Lactation on Bone Growth and Bone Health of Offspring

作　　者：韩立华 毛学英[1] HAN Lihua;MAO Xueying(College of Food Science and Nutritional Engineering/Key Laboratory of Functional Dairy,Ministry of Education,China Agricultural University,Beijing 100083,China;Culinary Science and Technology Department,Jiangsu College of Tourism,Yangzhou 225000,China)

机构地区：[1]中国农业大学食品科学与营养工程学院/教育部功能乳品重点实验室,北京100083 [2]江苏旅游职业学院烹饪科技学院,江苏扬州225000

出　　处：《食品科学技术学报》2024年第5期33-40,共8页Journal of Food Science and Technology

基　　金：国家自然科学基金资助项目(31871806);家畜产业技术体系北京市创新团队项目(BAIC05-2022)。

摘　　要：探究了母鼠补充乳脂肪球膜(milk fat globule membrane,MFGM)对其子代骨骼生长和骨骼健康的影响。在妊娠期和哺乳期对SD母鼠灌胃MFGM(400 mg/kg),子代出生后21 d时断奶,然后饲喂高脂饲料直至14周龄(成年子代),测定出生时(0 d)、出生后第10天和第21天断奶前子代骨骼纵向生长情况和成年子代(14周龄)的肥胖和骨健康情况。研究发现,母鼠妊娠期和哺乳期补充MFGM后,与对照组相比,断奶前子代的体质量、身长、尾长、股骨长度和胫骨长度均无显著变化,生长板增殖软骨细胞和肥大软骨细胞的高度较对照组无明显变化,增殖软骨细胞特异性蛋白Ⅱ型胶原(collagen type 2α,COL2α)和肥大软骨细胞特异性蛋白X型胶原(collagen type X,COLX)的表达均无显著差异。但是,母鼠妊娠期和哺乳期补充MFGM后,与对照组相比,成年子代由高脂饮食导致的体质量变化和白色脂肪组织积累均显著降低,骨髓腔脂肪细胞的数目显著减少,Runx2和OPG的蛋白表达显著上调,RANKL的蛋白表达显著降低。在母鼠妊娠期和哺乳期补充MFGM对断奶前子代的骨骼纵向生长无显著影响,但是可以有效缓解成年子代由高脂饮食导致的骨髓腔脂肪细胞积累,改善骨代谢进程。研究表明:母鼠补充MFGM可对成年子代的骨骼健康发挥长期保护作用。The effects of maternal milk fat globule membrane(MFGM)supplementation on skeletal growth and bone health of offspring were investigated.During pregnancy and lactation,SD female rats were orally administrated with MFGM at a dose of 400 mg/kg.Offspring were weaned at postnatal day 21 and subsequently fed a high-fat diet until postnatal week 14(adulthood).The longitudinal bone growth parameters of offspring were determined at birth(postnatal day 0),postnatal day 10 and 21.Obesity and bone health state of adult offspring rats were determined at postnatal week 14.Compared with the control group,no significant differences in body weight,body length,tail length,femur length and tibia length of offspring before weaning were found in maternal MFGM supplemented group.Similarly,there were no marked changes in the heights of proliferative and hypertrophic chondrocytes in the growth plate,nor in the expression levels of collagen type 2α(COL2α)and collagen type X(COLX),which are specific to proliferative and hypertrophic chondrocytes,respectively.However,maternal MFGM supplementation during pregnancy and lactation significantly reduced the body weight gain and accumulation of white adipose tissue caused by the high-fat diet in adult offspring compared to the control group.The number of bone marrow adipocytes was notably decreased,while the protein expression of Runx2 and OPG was upregulated significantly,and RANKL expression was downregulated significantly in the MFGM group.Although maternal MFGM supplementation had no significant effect on the longitudinal skeletal growth of pre-weaning offspring,it effectively mitigated the high-fat diet-induced accumulation of bone marrow adipocytes and improved bone metabolism in adult offspring.The study suggested that maternal supplementation with MFGM provided long-term protective effects on bone health of adult offspring.

关 键 词：乳脂肪球膜 生命早期 母亲营养 骨骼生长 骨骼健康 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]