男性肺癌患者尿液中小分子代谢标志物筛选  

Screening of Small Molecule Metabolite Markers in Urine of Male Lung Cancer Patients

作　　者：何佩宜 罗思文 祝曙光[2] 臧林泉 HE Peiyi;LUO Siwen;ZHU Shuguang;ZANG Linquan(College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou Guangdong,510006;Department of Cardiothoracic Surgery,the First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou Guangdong,510080)

机构地区：[1]广东药科大学药学院,广州510006 [2]广东药科大学附属第一医院胸心外科,广州510080

出　　处：《山西大同大学学报（自然科学版）》2024年第5期72-79,共8页Journal of Shanxi Datong University(Natural Science Edition)

基　　金：广东省自然科学基金[2017A030313856]。

摘　　要：目的从男性肺癌患者和健康成年人的尿液样本中筛选潜在的小分子肿瘤标志物,为肺癌的早期诊断和治疗提供科学依据。方法利用气相色谱质谱联用技术对13例男性肺癌患者和49例健康成年人的尿液样本进行代谢组学分析,并对图谱上的化合物信息进行统计分析,筛选匹配率和检出率较高的小分子代谢标志物。结果本研究共筛选出32种匹配率大于70%的代谢物,其中6种代谢物的检出率超过50%,分别为乙酸、氨基甲酸甲酯、2-羟基桉树脑/外-2-羟基桉树脑、2,3-蒎烷二醇、5-甲基海因和4-甲基苯酚。在健康成人尿液中,氨基甲酸甲酯和乙酸的检出率分别为89.79%和38.78%。结论这六种代谢物在肺癌患者中的检出率显著高于健康人群。其中,乙酸和氨基甲酸甲酯的检出率均达到92.31%,2-羟基桉树脑/外-2-羟基桉树脑和2,3-蒎烷二醇的检出率均达到76.92%。这些结果为后续的肿瘤标志物研究奠定了基础,对肺癌早期诊断具有潜在应用价值。Objective To screen and identify potential small molecule tumor markers from the urine samples of male lung cancer patients and healthy adults,providing a scientific basis for the early diagnosis and treatment of lung cancer.Methods Metabolomics analysis was performed on the urine samples of 13 male lung cancer patients and 49 healthy adults using gas chromatography-mass spectrometry(GC-MS).The compound information from the chromatograms was statistically analyzed to screen for small molecule metabolites with high match and detection rates.Results A total of 32 metabolites with a match percentage greater than 70%were identified,with six metabolites showing a detection rate greater than 50%.These metabolites include acetic acid,carbamic acid methyl ester,2-hydroxycineole/exo-2-hydroxycineole,2,3-pinanediol,5-methylhydantoin,and 4-methylphenol.In the urine of healthy adults,the detection rates of carbamic acid methyl ester and acetic acid were 89.79%and 38.78%,respectively.Conclusion The detection rates of these metabolites were significantly higher in lung cancer patients compared to the healthy population,with acetic acid and carbamic acid methyl ester both having a detection rate of 92.31%,and 2-hydroxycineole/exo-2-hydroxycineole and 2,3-pinanediol both having a detection rate of 76.92%.These findings lay the groundwork for subsequent research on tumor markers and suggest their potential application in the early diagnosis of lung cancer.

关 键 词：肺癌 尿液 小分子代谢物 代谢组学 

分 类 号：R734.2[医药卫生—肿瘤]