Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant.Part 2.Metabolomic profiling  

作　　者：James P.Meador Suzanne C.Ball C.Andrew James Jenifer K.McIntyre 

机构地区：[1]Dept.of Environmental and Occupational Health Sciences,School of Public Health,4225 Roosevelt Way NE,Suite 100,Seattle,WA,98105-6099,USA [2]Washington State University,School of the Environment,Puyallup Research and Extension Center,2606 W Pioneer Ave,Puyallup,WA,98371,USA [3]University of Washington Tacoma,Center for Urban Waters,326 East D Street,Tacoma,WA,98421-1801,USA

出　　处：《Aquaculture and Fisheries》2024年第3期367-377,共11页渔业学报（英文）

基　　金：support and guidance of the Wastewater Treatment Division of the King County Department of Natural Resources and Parks in addition to the King County Council for funding support(King County Contract#6113841).

摘　　要：Targeted metabolomic analysis was conducted on juvenile Chinook salmon exposed for 10 days to wastewater effluent(WWE)from a large urban treatment plant.Exposures included five dilutions of WWE(20%,5.3%,1.4%,0.4%,and 0.1%)and a control with 7 replicates per treatment.Liver was extracted from fish and analyzed via liquid chromatography–mass spectrometry(LC-MS)for 361 endogenous metabolites,of which 185 were detected.Control-versus-treatment comparisons identified several metabolites that were associated with altered biochemical pathways observed for all treatments,including several that are important for energy generation and utilization.These altered pathways are crucial for fish health and may be an early indicator of potential adverse effects on growth,reproduction,behavior,and immune function.Juvenile ocean-type Chinook salmon spend several days to weeks in the nearshore estuary where they may encounter high concentrations of WWE contaminants.They are exposed to a wide range of potent pharmaceuticals,personal care products,and industrial compounds from WWE that have the potential to affect physiological homeostasis and disrupt their normal life cycle.

关 键 词：Wastewater effluent Metabolomics PHYSIOLOGY Chinook salmon Pharmaceuticals Metabolic pathways ESTUARY 

分 类 号：X703[环境科学与工程—环境工程]