Prostaglandin E_(2) involvement in the reproduction of small abalone,Haliotis diversicolor  

作　　者：Yawei Shen Lingling Li Xuan Luo Miaoqin Huang Caihuan Ke Weiwei You Weidong Li 

机构地区：[1]State Key Laboratory of Marine Environmental Science,College of Ocean and Earth Sciences,Xiamen University,Xiamen,361102,China [2]Fujian Key Laboratory of Genetics and Breeding of Marine Organisms,Xiamen University,Xiamen,361102,China [3]School of Life Sciences,Xiamen University,Xiamen,361102,China [4]College of Ecology and Environment,Hainan University,Haikou,570228,China

出　　处：《Aquaculture and Fisheries》2024年第5期804-811,共8页渔业学报（英文）

基　　金：supported by grants from National Natural Science Foundation of China (No.31860728 and 32172961);Special Fund for Ocean and Fisheries of Xiamen (No.21CZY018HJ01).

摘　　要：Prostaglandin E2(PGE2)has been reported to be involved in reproduction as one of the most abundant and widely distributed prostaglandins in invertebrates.Regulating gonad development and gamete production in mollusks can greatly assist artificial breeding,which is believed to promote the commercial benefits of various mollusk species.In the present study,the contents of PGE2 in the gonads of abalone at different developmental stages(maturing stage,ripe stage,and spawning stage)and reproduction states(before and after spawning)were measured.A higher PGE2 level was found in the gonads of abalones at the ripe stage than other stages,and the highest PGE2 level was detected in the ovaries of female H.diversicolor in the UV-stimulated group.Then the effects of PGE2 injection on the spawning efficiency were explored,and the gamete emission rates of female and male abalone were significantly increased after PGE2 injection.Through qRT-PCR and in situ hybridization assay,higher expression levels and wide distribution of phospholipase A2(PLA2),cyclooxygenase 2(COX2),prostaglandin E synthases(PGES2),and prostaglandin E2 receptor 4(EP4)in the gonads of H.diversicolor were observed after spawning.It is speculated that PGE2 may promote the reproductive cycle in both male and female H.diversicolor,although the regulation mechanism of PGE2 in female and male abalone might be different.The results will be useful for the application of PGE2 in the abalone aquaculture practices to increase the reproduction efficiency in future.

关 键 词：PROSTAGLANDIN ABALONE REPRODUCTION GONAD 

分 类 号：S917[农业科学—水产科学]