FcγRIIB缺失促进DEN诱导小鼠肝细胞癌的发展  

FcγRIIB Deletion Promotes the Development of DEN-Induced Hepatocellular Carcinoma in Mice

作　　者：秦娣 秦学林[1,2] 丁雨欣 彭勇 QIN Di;QIN Xuelin;DING Yuxin;PENG Yong(School of Sports and Health,Nanjing Sport Institute,Nanjing 210014,China;Jiangsu Sports and Health Engineering Collaborative Innovation Center,Nanjing 210014,China;Key Laboratory of Human Sports Science for Jiangsu Province,Nanjing 210014,China)

机构地区：[1]南京体育学院运动健康学院,江苏南京210014 [2]江苏省运动与健康工程协同创新中心,江苏南京210014 [3]江苏省运动人体科学重点实验室,江苏南京210014

出　　处：《昆明理工大学学报（自然科学版）》2024年第5期133-138,共6页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：江苏省高校“青蓝工程”中青年学术带头人项目;江苏省高等学校大学生创新创业训练计划国家级项目(202410330003Z);南京体育学院“1+1”优秀学术团队项目(XSTD202317);江苏省运动与健康工程协同创新中心青年基金项目(JSIC-YP21001);南京体育学院重点实验室研究基金项目(SYS202101).

摘　　要：研究抑制性受体FcγRIIB在肝细胞癌(HCC)发生发展中的作用,为探讨肝脏炎癌转化的调控机制奠定基础.将FcγRIIB(129Sv)基因敲除鼠与C57BL/6小鼠进行杂交繁殖,通过PCR鉴定子代小鼠基因型;利用化学致癌剂DEN诱导小鼠HCC,比较FcγRIIB-/-小鼠和对照野生型(WT)小鼠的存活时间、肝脏肿瘤数量和肝脏重量指数,并用H&E染色观察两组小鼠肝细胞形态;采用聚乙二醇(PEG)沉淀比浊法比较两组小鼠血清中循环免疫复合物(CIC)水平.成功获得C57BL/6品系FcγRIIB缺失的纯合子小鼠即FcγRIIB-/-小鼠;在DEN诱导小鼠HCC模型中,FcγRIIB-/-小鼠的存活率显著低于WT小鼠,FcγRIIB-/-小鼠的肝脏肿瘤数量和肝脏重量指数均显著高于WT小鼠;H&E染色结果显示,FcγRIIB-/-小鼠肝细胞排列紊乱,异型性明显,见多核肿瘤细胞及炎性细胞浸润;PEG沉淀比浊法结果显示,FcγRIIB-/-小鼠血清中CIC含量显著高于WT小鼠.本研究提示FcγRIIB缺失能够促进DEN诱导小鼠HCC的发展,FcγRIIB可能在肝脏炎癌转化中发挥重要作用.This study assesses the role of inhibitory receptor FcγRIIB in the occurrence and development of HCC,which lays the foundation for exploring the regulatory mechanism of hepatic inflammation-induced carcinogenesis.FcγRIIB(129Sv)gene knockout mice were hybridized with C57BL/6 mice,and the genotype of the offspring mice was identified by PCR.The chemical carcinogen DEN was used to induce the mice HCC,the survival time,number of liver tumors,and liver weight index were compared between FcγRIIB-/-mice and WT mice.The morphology of hepatocytes in the two groups of mice was observed by H&E staining.The levels of CIC in the serum of two groups of mice were compared by PEG precipitation turbidimetry.The homozygous mice with FcγRIIB deletion of C57BL/6 strain(FcγRIIB-/-mice)were successfully obtained.In the DEN-induced mice HCC model,the survival rate of FcγRIIB-/-mice was significantly lower than that of WT mice,and the number of liver tumors and liver weight index in FcγRIIB-/-mice were significantly higher than those in WT mice.The results of H&E staining showed that the hepatocytes of FcγRIIB-/-mice were arranged in disorder with obvious heteromorphism,and multinuclear tumor cells and inflammatory cell infiltration were observed.The results of PEG precipitation turbidimetry showed that the levels of CIC in the serum of FcγRIIB-/-mice were significantly higher than that of WT mice.This study suggests that FcγRIIB deletion can promote the development of DEN-induced HCC in mice and FcγRIIB may play an important role in the hepatic inflammation-induced carcinogenesis.

关 键 词：抑制性受体FcγRIIB 肝细胞癌 循环免疫复合物 

分 类 号：R735.7[医药卫生—肿瘤]