枇杷叶提取物对高糖饮食诱导的黑腹果蝇代谢紊乱的缓解作用  

Alleviative effect of loquat leaf extract on metabolic disorders of Drosophila melanogaster induced by high sugar diet

作　　者：董翠[1] 孙安然 王家东[1] DONG Cui;SUN Anran;WANG Jiadong(School of Pharmacy,Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]信阳农林学院药学院,河南信阳464000

出　　处：《中国酿造》2024年第10期124-133,共10页China Brewing

基　　金：国家自然科学基金资助项目(12305399);河南省科技厅科技攻关项目(242102110243);信阳农林学院一流课程建设项目(YLBKKC-202335)。

摘　　要：为了探究枇杷叶提取物对高糖饮食诱导的代谢紊乱的缓解作用,以黑腹果蝇为研究对象,设置基础培养基、高糖培养基和分别添加1%、5%、10%枇杷叶提取物的含药培养基对黑腹果蝇进行培养,对各组黑腹果蝇的寿命、运动能力、生长发育指标、抗氧化能力、肠道完整性、肠道微生物多样性进行检测。结果表明,高糖饮食能造成黑腹果蝇代谢紊乱,在饮食中添加10%的枇杷叶提取物能通过延长黑腹果蝇寿命、提高老年黑腹果蝇的运动能力、提高黑腹果蝇卵的蛹化率和羽化率来提高黑腹果蝇的生长性能;通过提高黑腹果蝇体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,降低脂质过氧化物丙二醛(MDA)含量来提高黑腹果蝇的抗氧化能力;通过肠道菌群16S rRNA测序和分析发现,高糖饮食能导致黑腹果蝇肠道菌群失调,在饮食中添加10%枇杷叶提取物能通过提高菌群丰度、抑制肠炎相关菌群增殖、改变菌群组成等方式缓解菌群失调。结果显示,枇杷叶提取物可在一定程度上缓解高糖饮食造成的代谢紊乱。To investigate the alleviating effect of loquat leaf extract on metabolic disorders induced by high sugar diet,using Drosophila melanogaster as research object,D.melanogastriae was cultured with basic medium,high-sugar medium and medicated medium with 1%,5%and 10%loquat leaf extract,respectively,and their lifespan,locomotor ability,growth and developmental indices,antioxidant capacity,gut integrity and gut microbial diversity were tested in each group.The results showed that the high-sugar diet could cause metabolic disorders in D.melanogaster,and the addition of 10%loquat leaf extract to the diet could improve the growth performance of D.melanogaster by prolonging the lifespan of D.melanogastriae,improving the locomotor ability of older D.melanogaster,and increasing the pupation rate and feathering rate of D.melanogaster eggs.The antioxidant capacity of D.melanogaster was improved by increasing the activities of superoxide dismutase(SOD)and catalase(CAT)in D.melanogastriae and decreasing the levels of lipid peroxide malondialdehyde(MDA).Through 16S rRNA sequencing and analysis of gut flora,it was found that high-sugar diet could lead to intestinal flora dysbiosis in D.melanogaster,and the addition of 10%loquat leaf extract to the diet could alleviate intestinal flora dysbiosis by increasing the abundance of microflora,inhibiting the proliferation of enteritis-associated microflora,and changing the composition of microflora.The results indicated that loquat leaf extract could alleviate the metabolic disorders caused by high sugar diet to a certain extent.

关 键 词：枇杷叶 高糖饮食 代谢紊乱 肠道菌群多样性 

分 类 号：R285.5[医药卫生—中药学]