咀嚼力变化影响小鼠髁突发育及初级纤毛基因表达  

Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice

作　　者：扈梓悦 张帅 孙瑶[1] HU Ziyue;ZHANG Shuai;SUN Yao(Shanghai Engineering Research Center of Tooth Restoration and Regeneration&Tongji Research Institute of Stomatology&Department of Oral Implantology,Stomatological Hospital and Dental School,Tongji University,Shanghai 200072,China)

机构地区：[1]同济大学附属口腔医院口腔种植科,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学口腔医学研究所,上海200072

出　　处：《口腔颌面外科杂志》2024年第5期336-341,共6页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81822012);牛顿高级学者基金(82061130222)。

摘　　要：目的:探究咀嚼力改变后,髁突软骨发育及初级纤毛基因表达的变化。方法:取3周龄雄性C57BL/6J小鼠,将其随机分为3组,分别给予普通饲料(normal diet,ND)和软食(soft diet,SD)喂养2周,以及软食1周后恢复普通饲料喂养1周(re-normal diet,RND)。行番红O-固绿、甲苯胺蓝、Masson三色、碱性磷酸酶(alkaline phosphatase,ALP)、Ⅱ型胶原蛋白(collagen typeⅡ,COL2)免疫荧光染色,观察髁突软骨及软骨下骨的变化情况;实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-q PCR)检测初级纤毛相关基因Ift88、Ift140、Ptch1、Smo、Gli1的表达。结果:相较于ND组,SD组小鼠髁突软骨厚度降低,基质分泌减少,软骨下骨结构改变,初级纤毛相关基因Ift88、Ift140、Ptch1、Smo、Gli1的表达有下降趋势;相较于SD组,RND组上述变化有所恢复。结论:生长发育时期,咀嚼力变化可干扰髁突软骨的发育,同时影响初级纤毛相关基因表达。Objective:To explore the changes in condyle cartilage development and primary ciliary gene expression after changes in masticatory force.Methods:Three-week-old C57BL/6J male mice were divided into 3 groups,and they were fed with normal diet(ND)for 2 weeks,soft diet(SD)for 2 weeks,and fed with 1 week of soft diet than 1 week of re-normal diet(RND).After collecting the samples,safranin O-fast green staining,toluidine blue staining,Masson staining,alkaline phosphatase(ALP)staining,and collagen typeⅡ(COL2)immunofluorescence staining were performed to observe changes in the condylar cartilage and subchondral bone.Primary ciliary gene expression was detected by real-time quantitative polymerase chain reaction(RTqPCR).Results:Compared with the ND group,the SD group has thinner condyle cartilage,less extracellular matrix,and subchondral bone structure has changed.The primary cilia gene expression(Ift88,Ift140,Ptch1,Smo,Gli1)was decreased.Compared with the SD group,the above changes recovered in the RND group.Conclusion:Changes of masticatory force during growth will affect the development of condyle cartilage and primary ciliary gene expression in mice.

关 键 词：髁突软骨 咀嚼力 初级纤毛 

分 类 号：R782[医药卫生—口腔医学]