3株芽孢杆菌抗菌肽抑菌特性与抑菌谱研究  

Study on Antibacterial Peptide Characteristics and Antibacterial Spectrum of Three Strains of Bacillus

作　　者：余梦博 谢志恒 黎家明 林昊烨 袁志宁 顾万军 周淑贞 马驿[1] YU Meng-bo;XIE Zhi-heng;LI Jia-ming(Department of Veterinary Medicine,College of Coastal Agricultural Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524088;Foshan City Nanhai Eastern Along Pharmaceutical Co.,Ltd.,Foshan,Guangdong 528234)

机构地区：[1]广东海洋大学滨海农业学院动物医学系,广东湛江524088 [2]佛山市南海东方澳龙制药有限公司,广东佛山528234

出　　处：《安徽农业科学》2024年第20期88-91,126,共5页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金项目(2023A1515012181)。

摘　　要：[目的]分离筛选出有抑菌特性的芽孢杆菌,并研究其抑菌谱。[方法]从实验室分离鉴定的71株芽孢杆菌中筛选出对金黄色葡萄球菌和大肠杆菌抑菌性强的新种芽孢杆菌018株、贝莱斯芽孢杆菌F株和解淀粉芽孢杆菌221株,建立比较3株芽孢杆菌的遗传进化树,并应用酸沉淀法提取抗菌肽,比较3种芽孢杆菌抗菌肽提取物对11株动物病原菌的抑菌谱。[结果]3株芽孢杆菌产生的抗菌肽抑菌特性各有不同,其抑菌谱中3种芽孢杆菌抗菌肽提取物对试验菌株中的7株病原菌的抑菌性符合对指示菌的抑菌特性,对维氏气单胞菌、嗜水气单胞菌和猪传染性胸膜肺炎放线杆菌略有不同。[结论]芽孢杆菌可为开发应用抗菌肽的生物活性添加剂制剂或生物抗菌性药物提供参考。[Objective]The aim of this experiment was to isolate and screen Bacillus with antibacterial properties and study its antibacterial spectrum.[Method]From 71 strains of Bacillus spp.isolated and identified in the laboratory,we screened out new species of Bacillus 018,Bacillus velezensis F and Bacillus amyloliquefaciens 221,which are highly inhibitory to Staphylococcus aureus and Escherichia coli,the genetic evolution tree of 3 strains of Bacillus was established and compared,and the antimicrobial peptides were extracted by acid precipitation method.The antibacterial spectra of 3 strains of Bacillus against 11 strains of animal pathogens were compared.[Result]The results showed that the antimicrobial peptides produced by the three strains of Bacillus had different antibacterial properties.The antibacterial peptide extracts of the three strains in the antibacterial spectrum were in line with the antibacterial properties of the seven strains in the test strain against the indicator bacteria,but slightly different against Aeromonas vickerii,Aeromonas hydrophila and Actinobacillus transinfectious pleuropneumoniae.[Conclusion]The results show that Bacillus can provide reference for the development of bioactive additives or bioantibacterial drugs using antimicrobial peptides.

关 键 词：芽孢杆菌 抗菌肽 抑菌特性 抑菌谱 

分 类 号：S816.3[农业科学—饲料科学]