有机肥对烟叶次生代谢物影响分析  

Analysis of the Effect of Organic Fertilizer on Secondary Metabolites in Tobacco Leaves

作　　者：申树林 李洪勋 黄兰 张昆鹏[5] 朱勇 张龙 陈德慧 尹能文 曲存民[1] 彭华伟 林叶春 SHEN Shu-lin;LI Hong-xun;HUANG Lan(College of Agronomy and Biotechnology,Southwest University,Chongqing 400715;Upland Flue-Cured Tobacco Quality&Ecology Key Laboratory of China Tobacco,Guiyang,Guizhou 550081;Guizhou Academy of Tobacco Sciences,Guiyang,Guizhou 550081;Bijie Branch of Guizhou Tobacco Company,Bijie,Guizhou 551700)

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]山地烤烟品质与生态重点实验室,贵州贵阳550081 [3]贵州省烟草科学研究院,贵州贵阳550081 [4]贵州省烟草公司毕节市公司,贵州毕节551700 [5]江苏中烟工业有限责任公司,江苏南京210019

出　　处：《安徽农业科学》2024年第20期141-144,154,共5页Journal of Anhui Agricultural Sciences

基　　金：贵州省烟草公司毕节市公司2021年科技项目(20215205-00240048)。

摘　　要：肥料是影响烟叶产量、品质的重要因素之一,其中施用有机肥可促进烟叶增产,改善烟叶品质。为探究增施有机肥对烟叶代谢的影响,以云烟87为材料,通过增施0、900和1500 kg/hm^(2)3个不同梯度的炭基(酒糟)有机肥处理后,利用液相色谱质谱方法对打顶后7 d烟叶中的代谢物进行了非靶向代谢组分析。在烟叶中鉴定了219种次生代谢物,通过代谢物比较分析发现,增施900和1500 kg/hm^(2)分别有14和26个代谢物相比对照组积累降低;有7种代谢物在2个处理组中均显著降低,其中包含了M780(降烟碱)、M1021(尼古丁)和M1328(可替宁)3种烟碱类代谢物。进一步分析发现,33种差异代谢物中有9种代谢物在增施900和1500 kg/hm^(2)2个分组之间具有积累差异;其余差异代谢物在2个分组之间无显著积累差异。差异代谢物相关网络分析发现,山奈酚等苯丙烷途径代谢物与烟碱的积累变化紧密相关,也具有降低的趋势。结果表明,增施有机肥既能显著减少烟叶烟碱类代谢物的积累,也能在一定程度上降低烟叶苯丙烷途径代谢物的积累;增施900和1500 kg/hm^(2)有机肥对烟碱的影响无明显差异。综上,明确了有机肥对烟叶次生代谢物的影响,为后续烟叶生产中有机肥合理施用提供了一定的理论指导。Fertilizer is one of the important factors affecting the yield and quality of tobacco leaves,among which the application of organic fertilizer can promote the increase of tobacco leaf yield and improve the quality of tobacco leaves.To explore the effect of increasing the application of organic fertilizers on the metabolism of tobacco leaves,Yunyan 87 was used as the material in this study.After applying three different gradients of carbon-based organic fertilizers at 0,900 and 1500 kg/hm^(2),and by using the liquid chromatography-mass spectrometry method,the metabolites in tobacco leaves 7 days after topping were subjected to untargeted metabolome analysis.A total of 219 secondary metabolites were identified in tobacco leaves.Through comparative analysis of metabolites,it was found that 14 and 26 metabolites were reduced in the accumulation of 900 and 1500 kg/hm^(2) respectively compared with the control group;7 metabolites in the two treatments were significantly reduced,which contained three nicotinic metabolites M780(nornicotine),M1021(nicotine)and M1328(cotinine).Further analysis found that 9 of the 33 differential metabolites had accumulation differences between the two groups with additional application of 900 and 1500 kg/hm^(2);the rest of the differential metabolites had no significant accumulation differences between the two groups.Correlation network analysis of differential metabolites found that phenylpropane pathway metabolites such as kaempferol were closely related to the accumulation changes of nicotine,and also had decreased trend.The above results showed that increasing the application of organic fertilizers could not only significantly reduce the accumulation of nicotinic metabolites in tobacco leaves,but also reduce the accumulation of metabolites in the phenylpropanoid pathway in tobacco leaves to a certain extent;there was no significant difference between adding 900 and 1500 kg/hm^(2) organic fertilizer on nicotine.In summary,this study clarified the effects of organic fertilizers on second

关 键 词：有机肥 烟叶 代谢物 烟碱 类黄酮 

分 类 号：S572[农业科学—烟草工业]