氨基酸组成及分子表面属性对胶原与盘状结构域受体DDR2结合能力的影响  

Effects of Amino Acid Composition and Molecular Surface Properties on the Binding Ability of Collagen to Discoidin Domain Receptor 2

作　　者：韩庆秋 寇慧芝 未本美 许承志 侯袁静 汪海波 HAN Qingqiu;KOU Huizhi;WEI Benmei;XU Chengzhi;HOU Yuanjing;WANG Haibo(School of Chemical and Environmental Engineering,Wuhan Polytechnic University,Wuhan 430023,China;College of Life Science and Technology,Hubei Engineering University,Xiaogan 432000,Hubei,China)

机构地区：[1]武汉轻工大学化学与环境工程学院,武汉430023 [2]湖北工程学院生命科学与技术学院,湖北孝感432000

出　　处：《材料导报》2024年第20期265-271,共7页Materials Reports

基　　金：国家自然科学基金(22178277)。

摘　　要：本工作利用ELISA实验和细胞黏附实验研究了不同物种来源的胶原样本与盘状结构域受体DDR2及人舌鳞癌细胞CAL-27的结合能力,并进一步探讨了氨基酸组成对胶原与细胞受体DDR2结合能力的影响。结果表明,不同来源的胶原样本与DDR2及CAL-27细胞的结合能力存在显著性差异,其中,哺乳动物胶原的结合能力明显大于鱼类胶原,各鱼类胶原之间也存在差异性。通过分析胶原蛋白的氨基酸组成与胶原-受体DDR2结合能力之间的相关性可知,胶原-DDR2结合能力与天冬氨酸、苏氨酸、谷氨酸、甘氨酸、缬氨酸、酸性氨基酸、带电荷极性氨基酸、非极性氨基酸呈负相关,与羟脯氨酸、亚氨基酸、羟基化率(%)、不带电荷极性氨基酸、总极性氨基酸呈正相关(P<0.05)。与此同时,进一步研究了胶原蛋白分子的表面属性对胶原-受体DDR2结合能力的影响。分析表明,较低的Zeta电位绝对值或较高的亲水性均有助于胶原与DDR2的结合。The binding ability of collagen samples from different species to discoidin domain receptor 2 and human tongue squamous cell CAL-27 was stu-died by ELISA and cell adhesion experiments,and the effect of amino acid composition on the binding ability of collagen to cell receptor DDR2 was further investigated.The results showed that there were significant differences in the binding ability of collagen samples from different sources to DDR2 and CAL-27 cells.Among them,the binding ability of mammalian collagen was significantly greater than that of fish collagen,and there were also differences among fish collagen.The correlation analysis between amino acid composition and collagen-receptor DDR2 bin-ding ability showed that collagen-DDR2 binding ability was negatively correlated with aspartate,threonine,glutamic acid,glycine,valine,acidic amino acid,charged polar amino acid and non-polar amino acid.It was positively correlated with hydroxyproline,amino acid,hydroxylation rate(%),uncharged polar amino acid and total polar amino acid(P<0.05).At the same time,the influence of collagen molecular surface properties,including Zeta potential and hydrophilicity,on its binding ability to DDR2 receptor was further studied.Analysis showed that lower Zeta potential absolute values or higher hydrophily contribute to the binding of collagen to DDR2.

关 键 词：胶原蛋白 盘状结构域受体DDR2 氨基酸 结合能力 

分 类 号：O636.9[理学—高分子化学]