亚临界萃取法辅助探究碱提法不同因素对提取黑水虻蛋白的影响  

Investigating the Effects of Different Factors on the Extraction of Black Soldier Fly(Hermetia illucens L.)Protein by Alkaline Extraction Method based on Subcritical Extraction Assistance

作　　者：郑朝中 王飞[1] 丘嘉诚 王会 章启慧 陈玉梁 王雷雨 庄志凯 Zheng Chaozhong;Wang Fei;Qiu Jiacheng;Wang Hui;Zhang Qihui;Chen Yuliang;Wang Leiyu;Zhuang Zhikai(Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524001,China;Guangdong Ocean University,Zhanjiang 524088,China;Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]中国热带农业科学院农产品加工研究所,广东湛江524001 [2]广东海洋大学,广东湛江524088 [3]江西农业大学,江西南昌330045

出　　处：《广东化工》2024年第19期188-191,共4页Guangdong Chemical Industry

基　　金：海南省自然科学基金青年基金项目(321QN304,323QN290);中国热带农业科学院2022年科技“揭榜挂帅”项目(1630122022008);中国热带农业科学院基本科研业务费专项项目(1630122023007)。

摘　　要：本研究通过亚临界萃取技术对黑水虻(Hermetia illucens L.)幼虫粉进行脱脂处理,并对脱脂黑水虻幼虫粉进行4种方法的蛋白提取,确定最优法后进一步探究最优提取方法的各种因素下的最适水平。亚临界萃取技术采用丁烷为溶剂、温度45℃、压力0.4 MPa、时间40 min·次^(-1)、萃取3次,得到脱脂率为87.09%的脱脂黑水虻幼虫粉,4种提取方法的蛋白提取率分别为:碱提法(53.17±2.97%)、胰蛋白酶提法(5.40±0.36%)、盐提法(5.13±0.84%)和Tris-HCl缓冲液提法(7.27±0.40%),探究碱提法的4种因素下的最适水平,单因素试验结果分别为NaOH溶液浓度(25 g·L^(-1)),浸提时间(1.5 h),浸提温度(60℃),液料比(25 mL·g^(-1))。In this study,the black soldier fly(Hermetia illucens L.)larva powder was defatted by subcritical extraction technique,and the protein extraction of defatted black soldier fly larva powder was carried out by four methods,and the optimal method was determined and then further explored the optimal level of the optimal extraction method under various factors.The subcritical extraction technique used butane as solvent,temperature 45℃,pressure 0.4 MPa,40 min/time,and extraction 3 times to obtain the defatted black soldier fly larva powder with 87.09%defatted rate.The protein extraction rates of the four extraction methods were as follows:alkaline extraction method(53.17±2.97%),trypsin extraction method(5.40±0.36%),salt extraction method(5.13±0.84%),and Tris-HCl buffer extraction method(7.27±0.40%).To explore the optimum level of alkaline extraction method under four factors,the results of the one-way test were the concentration of NaOH solution(25 g·L^(-1)),the extraction time(1.5 h),the temperature of the extraction(60℃),and the liquid-feed ratio(25 mL·g^(-1)).

关 键 词：亚临界萃取 黑水虻 蛋白提取 碱提法 单因素试验 

分 类 号：TQ89[化学工程]