抗奶牛乳房炎主要病原菌的乳酸菌细菌素理化特性研究  

Physicochemical Properties of Lactic Acid Bacteria Bacteriocin against Main Pathogenic Bacteria of Cow Mastitis

作　　者：李琪瑶 张涵 涂会鑫 华文静 董文龙 LI Qiyao;ZHANG Han;TU Huixin;HUA Wenjing;DONG Wenong(College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Jilin,Jilin 132109,China)

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132109

出　　处：《家畜生态学报》2024年第10期66-71,共6页Journal of Domestic Animal Ecology

基　　金：吉林农业科技学院博士启动基金项目(20215021)。

摘　　要：该研究旨在对一株产抗奶牛乳房炎主要病原菌抗菌活性物质的菌株LQY-4进行菌种鉴定,并对其所分泌抗菌活性物质的生物学特性进行初步研究,为治疗奶牛乳房炎研发新型替抗产品奠定良好基础。采用形态学、生化试验以及16S rRNA基因序列测定等方法对菌株进行鉴定。以大肠杆菌为指示菌,采用琼脂扩散法确定其发酵上清液的抗菌活性,并对抗菌物质进行蛋白酶敏感性试验、热处理以及酸碱处理试验确定其抗菌活性物质生物学特性,最后进行抑菌谱的测定。结果表明:所分离菌株LQY-4为植物乳杆菌,其发酵上清液对奶牛乳房炎主要病原菌(大肠杆菌、金黄色葡萄球菌、化脓隐秘杆菌)具有良好抗菌活性,且对蛋白酶具有高度敏感性,推测其初提物中具有抗菌活性的物质为一种细菌素。研究结果说明植物乳杆菌LQY-4所产细菌素具有较好的热稳定性及酸稳定性,在代替抗生素治疗奶牛乳房炎方面具有良好的应用前景。The aim of this study was to identify the bacteria strain LQY-4 which produces antibacterial active substances against the main pathogens causing mastitis in dairy cows,and to conduct a preliminary investigation into the biological characteristics of the antibacterial substances it secretes,in order to lay a solid foundation for the development of novel alternatives for treating mastitis in dairy cows.Strains were identified by morphological,biochemical tests and 16S rRNA gene sequencing.Escherichia coli as the indicator bacteria,the agar well diffusion method was used to determine the antibacterial activity of the fermented supernatant,and protease susceptibility test,heat treatment as well as acid-base treatment test were performed to determine the biological characteristics of the antibacterial active substances,and finally,the determination of the inhibition spectrum was performed.Results showed that the isolated strain LQY-4 was identified as Lactiplantibacillus plantarum,and its fermented supernatant exhibited good antibacterial activity against major pathogens of mastitis(Escherichia coli,Staphylococcus aureus,Trueperella pyogenes).Moreover,it showed high sensitivity to proteases,so that the substance with antibacterial activity in its primary extract was assumed to be a bacteriocin.In conclusion,the substance of L.plantarum LQY-4 demonstrates better thermal and acid stability,making it a promising alternative to antibiotics for the treatment of mastitis in dairy cows.

关 键 词：奶牛乳房炎 病原菌 细菌素 替抗 

分 类 号：S852.61[农业科学—基础兽医学] S858.23[农业科学—兽医学]