山葡萄VaJAZ9和VaCOI1基因的抗寒功能研究  

Study on the Cold Resistance Function of VaJAZ9 and VaCOI1 Genes in Vitis amurensis

作　　者：孙雨桐 刘德帅 黄栩筝 迟敬楠 齐迅 姚文孔 SUN Yutong;LIU Deshuai;HUANG Xuzheng;CHI Jingnan;QI Xun;and YAO Wenkong(College of Enology and Horticulture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学葡萄酒与园艺学院,银川750021

出　　处：《园艺学报》2024年第10期2243-2254,共12页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31960586);宁夏回族自治区农业育种项目(NXNYYZ202101)。

摘　　要：以山葡萄(Vitis amurensis Rupr.)‘左山–1’为试材,克隆了VaCOI1基因并对其功能进行分析。结果表明,VaCOI1基因位于13号染色体,其ORF全长1797 bp,编码598个氨基酸,属于F-box家族基因;聚类分析表明VaCOI1蛋白与锦葵HuCOI1和可可TcCOI1同源性较高。双分子荧光互补实验表明VaJAZ9与VaCOI1蛋白具有互作关系。对异源过表达VaJAZ9和VaCOI1拟南芥株系进行低温处理,过表达VaCOI1拟南芥的成活率高于野生型,电导率低于野生型,而过表达VaJAZ9拟南芥的成活率低于野生型,电导率高于野生型。实时荧光定量PCR分析表明,过表达VaCOI1拟南芥中与低温相关的基因AtKIN1、AtCOR15A及AtRD29A表达量上调,而过表达VaJAZ9植株中AtKIN1、AtCOR15A及AtRD29A表达量下调。综上所述,VaCOI1在低温胁迫反应中起正调控的作用,而VaJAZ9起负调控的作用。In this study,the VaCOI1 gene from Vitis amurensis Rupr.‘Zuoshan-1’were cloned,and the gene function was analysed.The results show that the VaCOI1 gene is located on chromosome 13,and its ORF has a full-length of 1797 bp,encoding 598 amino acids,and belongs to the F-box family.Clustering analysis indicates that the VaCOI1 protein performed a high homology with HuCOI1(Herrania umbratica)and TcCOI1(Theobroma cacao).Bimolecular fluorescence complementation(BiFC)assays indicate that VaJAZ9 can interact with the VaCOI1 protein.The heterologously overexpressing of VaJAZ9 and VaCOI1 in Arabidopsis thaliana shows that at the low temperature,the VaCOI1 overexpressing plants had a higher survival rate and a lower conductivity than the wild type plants,whereas the VaJAZ9 overexpressing plants had a lower survival rate and a higher conductivity than the wild type plants.Quantitative real-time PCR(qRT-PCR)analysis show that the low temperature related genes including AtKIN1,AtCOR15A and AtRD29A were up-regulated in the VaCOI1 overexpressing A.thaliana,whereas these genes were down-regulated in overexpressing VaJAZ9 plants.In conclusion,the results show that the VaCOI1 acts as a positive regulator in response to low temperature stress,whereas the VaJAZ9 acts as a negative regulator.

关 键 词：山葡萄 茉莉酸信号途径 JAZ9 COI1 抗寒 

分 类 号：S663.1[农业科学—果树学]