LINC00852调节miR-671-5p/MYH9信号轴对非小细胞肺癌细胞增殖侵袭及迁移的影响  

Effect of LINC00852 Regulating the miR-671-5p/MYH9 Signaling Axis on Proliferation Invasion and Migration of Non-Small Cell Lung Cancer Cells

作　　者：李亚明[1] 张进召[1] 刘松 潘双 刘佳银 LI Yaming;ZHANG Jinzhao;LIU Song(The First Affiliated Hospital of Xi'an Medical University,Shaanxi Xi'an 710077,China)

机构地区：[1]西安医学院第一附属医院呼吸与危重症医学科,陕西西安710077

出　　处：《河北医学》2024年第10期1585-1591,共7页Hebei Medicine

基　　金：陕西省重点研发计划项目,(编号:2022SF-554)。

摘　　要：目的:探讨长链非编码RNA00852(LINC00852)调节微小RNA-671-5p(miR-671-5p)/肌球蛋白重链9(MYH9)信号轴对非小细胞肺癌(NSCLC)细胞增殖、侵袭及迁移的影响。方法:采用qRT-PCR法检测45例2022年9月至2023年12月期间在我院进行手术的NSCLC患者术中切除的NSCLC组织及癌旁组织中LINC00852、miR-671-5p、MYH9 mRNA的表达。以A549细胞为研究对象,将其随机分为Control组、sh-NC组、sh-LINC00852组、sh-LINC00852+anti-NC组、sh-LINC00852+anti-miR-671-5p组。检测各组中LINC00852、miR-671-5p、MYH9 mRNA的表达;平板克隆实验、Transwell实验、划痕实验分别检测敲低LINC00852对A549细胞的增殖、侵袭、迁移的影响;Western blot检测各组A549细胞中PCNA、MYH9、MMP-9蛋白的表达。双荧光素酶报告基因实验检测LINC00852与miR-671-5p、miR-671-5p与MYH9之间的互作。结果:NSCLC组织LINC00852(1.65±0.34)、MYH9 mRNA表达(1.73±0.35)高于癌旁组织[(0.98±0.29)、(1.01±0.33)](t=10.058、10.041,P<0.05),miR-671-5p表达(0.52±0.15)低于癌旁组织(1.00±0.18)(t=13.742,P<0.05)。sh-LINC00852组A549细胞的克隆数、侵袭数、划痕愈合率、LINC00852、MYH9 mRNA和蛋白、PCNA蛋白、MMP-9蛋白表达低于sh-NC组、Control组,miR-671-5p表达高于sh-NC组、Control组(P<0.05);与sh-LINC00852组、sh-LINC00852+anti-NC组相比,sh-LINC00852+anti-miR-671-5p组克隆数、侵袭数、划痕愈合率、MYH9 mRNA和蛋白、PCNA蛋白、MMP-9蛋白表达升高,miR-671-5p表达降低(P<0.05)。LINC00852可以靶向负调控miR-671-5p,miR-671-5p可以靶向负调控MYH9。结论:敲低LINC00852可以抑制NSCLC细胞的增殖、侵袭、迁移,其机制可能是通过调控miR-671-5p/MYH9信号通路实现的。Objective:To investigate the effects of long non-coding RNA 00852(LINC00852)on the proliferation,invasion,and migration of non-small cell lung cancer(NSCLC)cells by regulating the microRNA-671-5p(miR-671-5p)/myosin heavy chain 9(MYH9)signaling axis.Methods:The expression levels of LINC00852,miR-671-5p,and MYH9 mRNA were detected in NSCLC tissues and adjacent normal tissues using qRT-PCR.A549 cells were randomly divided into several groups:Control group,sh-NC group,sh-LINC00852 group,sh-LINC00852+anti-NC group,and sh-LINC00852+anti-miR-671-5p group.The expression levels of LINC00852,miR-671-5p,and MYH9 mRNA were detected.Cell proliferation,invasion,and migration were assessed by colony formation assay,Transwell assay,and wound healing assay,respectively.Western blot was used to detect the protein expression of PCNA,MYH9,and MMP-9.Dual-luciferase reporter assay was performed to verify the interaction between LINC00852 and miR-671-5p,and between miR-671-5p and MYH9.Results:The expression levels of LINC00852 and MYH9 mRNA were significantly higher in NSCLC tissues compared with adjacent normal tissues,while the expression of miR-671-5p was significantly lower.Knockdown of LINC00852 inhibited the proliferation,invasion,and migration of A549 cells,and downregulated the expression of MYH9,PCNA,and MMP-9,while upregulating the expression of miR-671-5p.Dual-luciferase reporter assay confirmed that LINC00852 could directly target miR-671-5p,and miR-671-5p could directly target MYH9.Conclusion:LINC00852 inhibits the proliferation,invasion,and migration of NSCLC cells by regulating the miR-671-5p/MYH9 signaling axis.

关 键 词：长链非编码RNA00852 微小RNA-671-5p 肌球蛋白重链9 非小细胞肺癌 增殖 侵袭 迁移 

分 类 号：R734.2[医药卫生—肿瘤]