DNMT3B与乳腺癌预后的关系及其生物学机制  

Relationship between DNMT3B and prognosis of breast cancer and its biological mechanism

作　　者：张志兆 王睿 郜苹苹 王成方 王成 齐晓伟 Zhang Zhizhao;Wang Rui;Gao Pingping;Wang Chengfang;Wang Cheng;Qi Xiaowei(Department of Breast and Thyroid Surgery,Southwest Hospital,Army Medical University,Chongqing 400038,China)

机构地区：[1]陆军军医大学西南医院乳腺甲状腺外科,重庆400038

出　　处：《中华普外科手术学杂志（电子版）》2024年第6期624-629,共6页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

基　　金：重庆市杰出青年自然科学基金:难治性乳腺癌耐药机制研究(CSTB2023NSCQ-JQX0012)。

摘　　要：目的分析DNA甲基转移酶3B(DNMT3B)在乳腺癌中的表达及其对预后的影响,探究其在乳腺癌发生发展中及潜在靶点作用。方法通过R语言程序分析TCGA乳腺癌数据库,使用UALCAN、Starbase database、cBioPortal、GeneMANIA、STRING等在线数据库分析DNMT3B在乳腺癌中的表达、诊断和预后价值、肿瘤免疫微环境、GO功能、KEGG信号通路和蛋白相互作用。采用qRT-PCR检测DNMT3B在常见乳腺癌细胞系和正常乳腺上皮细胞间的表达差异。结果在线数据库分析及验证实验提示DNMT3B在乳腺癌中高表达,且其高表达不利于乳腺癌患者的生存,提示DNMT3B可能具有诊断价值。通过GO功能、KEGG信号通路和蛋白相互作用以及肿瘤免疫微环境分析,提示DNMT3B参与乳腺癌发生发展的多种机制,可能成为新的治疗靶点。结论DNMT3B能促进乳腺癌的发生并影响疾病的进展,可能是乳腺癌潜在的肿瘤标志物及治疗靶点。Objective To analyze the expression of DNA methyltransferase 3B(DNMT3B)in breast cancer and its influence on prognosis,and explore its role in the occurrence and development of breast cancer and potential targets.Methods Analyzing TCGA breast cancer database through R language program,Online databases such as UALCAN,Starbase database,cBioPortal,GeneMANIA and STRING were used to analyze DNMT3B expression,diagnostic and prognostic value,tumor immune microenvironment,GO function,KEGG signaling pathway and protein interaction in breast cancer.The expression difference of DNMT3B between common breast cancer cell lines and normal breast epithelial cells was detected by QRT-PCR.Results Online database analysis and validation experiments suggest that DNMT3B is highly expressed in breast cancer,and its high expression is not conducive to the survival of breast cancer patients,suggesting that DNMT3B may have diagnostic value.Through GO function,KEGG signaling pathway and protein interaction,and tumor immune microenvironment analysis,DNMT3B is involved in multiple mechanisms of breast cancer occurrence and development,and may become a new therapeutic target.Conclusion DNMT3B can promote the occurrence of breast cancer and affect the progression of the disease,and may be a potential tumor marker and therapeutic target for breast cancer.

关 键 词：乳腺肿瘤 DNA甲基转移酶3B 肿瘤免疫微环境 预后 

分 类 号：R737.9[医药卫生—肿瘤]