HSP90α在宫颈癌细胞及组织中的表达及其与肿瘤凋亡的关系  

Expression of HSP90αin cervical cancer cells and tissues and its relationship with tumor apoptosis

作　　者：刘金钰 邓萍[1] 石磊[1] 何茂旭 LIU Jinyu;DENG Ping;SHI Lei;HE Maoxu(Jilin Cancer Hospital,Changchun 130012,China)

机构地区：[1]吉林省肿瘤医院,吉林长春130012

出　　处：《中国实验诊断学》2024年第10期1165-1170,共6页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省社会发展科技项目(20240304083SF)。

摘　　要：目的探讨分析HSP90α在宫颈癌细胞及组织中的表达及其与肿瘤凋亡的关系。方法选择2019年4月至2021年4月吉林省肿瘤医院收治的103例宫颈癌患者,取其术中切除癌组织及癌旁组织,采用RT-qPCR法检测组织中HSP 90αmRNA相对表达量,并分析HSP 90α基因表达与患者临床病理特征及无进展生存预后的关系。采用RT-qPCR检测宫颈癌HeLa、CaSki、C-33A细胞及正常宫颈上皮细胞End1/E6E7中HSP90αmRNA水平表达。采用HeLa细胞,将其分为空白对照组、shRNA组及sh-NC组,检测各组细胞中HSP90αmRNA表达水平,采用CCK8法检测各组细胞增殖情况,采用流式细胞技术检测各组细胞凋亡情况,采用Western Blot法检测各组细胞凋亡相关基因表达情况。结果宫颈癌患者癌组织HSP 90αmRNA相对表达量显著高于癌旁组织(P<0.05)。癌组织HSP 90αmRNA表达与患者浸润深度、临床分期及淋巴结转移有关(P<0.05)。HSP90α低表达组患者无进展生存预后显著优于高表达组(P<0.05)。宫颈癌HeLa、CaSki、C-33A细胞株HSPαmRNA相对表达量显著高于正常宫颈上皮细胞株End1/E6E7(P<0.05)。shRNA组细胞HSP90αmRNA相对表达量显著低于空白对照组及sh-NC组(P<0.05)。24、48、72、96 h时,shRNA组细胞450 nm处吸光度值显著低于空白对照组及sh-NC组(P<0.05)。shRNA组细胞凋亡率显著高于空白对照组及sh-NC组(P<0.05)。shRNA组细胞Caspase-3、Bax蛋白相对表达量较空白对照组、sh-NC组显著升高(P<0.05),shRNA组细胞Bcl-2、Cyclin D1蛋白相对表达量较空白对照组、sh-NC组显著降低(P<0.05)。结论在宫颈癌组织及细胞中,均存在HSP90α高表达,HSP90α参与宫颈癌的发生发展过程,抑制宫颈癌细胞中HSP90α表达,可有效抑制肿瘤增殖活性,激活细胞凋亡通路而促进细胞凋亡,HSP90α有望成为宫颈癌治疗的潜在生物标志物。Objective To investigate the expression of HSP90αin cervical cancer cells and tissues and its relationship with tumor apoptosis.Methods 103patients with cervical cancer admitted to our hospital from April 2019to April 2021were selected,and the cancerous and adjacent tissues were removed during surgery.The relative expression of HSP 90αmRNA in the tissues was detected by RT-qPCR method,and the relationship between the expression of HSP 90αgene and the clinical pathological features and progression-free survival of the patients was analyzed.RT-qPCR was used to detect the expression level of HSP90αin HeLa,CaSki,C-33Acervical cancer cells and normal cervical epithelial cells End1/E6E7.Using HeLa cells,they were divided into blank control group,shRNA group,and sh-NC group,and the expression levels of HSP90αmRNA in each group of cells were detected.The proliferation of each group of cells was detected by CCK8method,and the apoptosis of each group of cells was detected by flow cytometry.The expression of apoptosis-related genes in each group of cells was detected by Western Blot method.Results The relative expression of HSP 90αmRNA in cervical cancer tissues was significantly higher than that in adjacent tissues(P<0.05).The expression of HSP90αmRNA in cancer tissue is related to the depth of invasion,clinical stage,and lymph node metastasis of the patients(P<0.05).The progression-free survival of patients with low expression of HSP90αwas significantly better than that of patients with high expression(P<0.05).The relative expression levels of HSPαmRNA in cervical cancer cell lines HeLa,CaSki,and C-33Awere significantly higher than those in normal cervical epithelial cell line End1/E6E7(P<0.05).The relative expression of HSP90αmRNA in shRNA group was significantly lower than that in the blank control group and sh-NC group(P<0.05).At 24h,48h,72h,and 96h,the absorbance at 450nm in the shRNA group was significantly lower than that of the blank control group and the sh-NC group(P<0.05).The cell apoptosis rate in the

关 键 词：HSP90Α 宫颈癌细胞 肿瘤凋亡 

分 类 号：R737.33[医药卫生—肿瘤]