NF-κB抑制剂APDC对人皮肤基底细胞癌迁移的影响  

The migration effect of NF-κB inhibitor APDC on human skin basal cell carcinoma

作　　者：于景云 周赵茹 季海伟 胜金金 耿雨佳 王浩天[1] 金洪娟[2] YU Jingyun;ZHOU Zhaoru;JI Haiwei;SHENG Jinjin;GENG Yujia;WANG Haotian;JIN Hongjuan(School of Pharmaceutical Sciences Jilin University,Changchun 130021,China;Department of Plastic and Reconstructive Surgery,the First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学药学院,吉林长春130021 [2]吉林大学第一医院整形外科,吉林长春130021

出　　处：《中国实验诊断学》2024年第10期1214-1218,共5页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技发展计划项目(自然科学基金20200201402JC)。

摘　　要：目的探究NF-κB抑制剂APDC对人皮肤基底细胞癌(BCC)的迁移影响及机制。方法用MTT法检测NF-κB抑制剂APDC对人BCC细胞增殖活性的影响。通过细胞划痕和Transwell侵袭实验观察应用NF-κB抑制剂后BCC细胞生长特性的变化,应用RT-qPCR检测不同浓度APDC抑制剂对TE354.T细胞中NF-κB mRNA表达情况的影响,最后通过免疫印迹法探究对细胞中NF-κB蛋白表达的影响。结果NF-κB抑制剂APDC对TE354.T细胞的增殖有抑制作用,且呈剂量依赖性,有效抑制TE354.T细胞的迁移能力和侵袭能力,随时间呈剂量依赖性,同时对TE354.T细胞NF-κB mRNA水平有抑制作用,呈正相关,且NF-κB蛋白表达有所降低。结论NF-κB抑制剂APDC能够抑制BCC的细胞增殖,影响BCC NF-κB蛋白和mRNA水平表达,且有效抑制皮肤基底细胞癌的迁移和侵袭能力。Objective To explore the migration effect of NF-κB inhibitor APDC on human skin basal cell carcinoma and its mechanism.Methods To detect of the effect of NF-κB inhibitor APDC on the proliferation activity of human BCC cells using MTT assay.Observation of changes in the growth characteristics of BCC cells after the application of NF-κB inhibitor through cell scratch and Transwell invasion experiment,Application of RT-qPCR to detect the effect of different concentrations of APDC inhibitor on NF-κB mRNA expression in TE354.T cells,finally,the impact on protein expression was explored through Western blot.Results NF-κB inhibitor APDC has an inhibitory effect on the proliferation of TE354.T cells in a dose-dependent manner,APDC effectively inhibits the migration and invasion ability of TE354.T cells in a dose-dependent manner over time,at the same time,APDC has an inhibitory effect on the mRNA level of TE354.T cells,showing apositive correlation and reducing protein expression.Conclusion NF-κB inhibitor APDC can inhibit cell proliferation of BCC,affect the expression of BCC NF-κB protein and mRNA levels,and effectively inhibit the migration and invasion ability of skin basal cell carcinoma.

关 键 词：NF-ΚB 皮肤基底细胞癌 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R739.5[医药卫生—肿瘤]