蜡样芽孢杆菌T4对TNT的降解及耐受机制  

Analysis of TNT Degradation and Tolerance Mechanism of Bacillus cereus Strain T4

作　　者：尹茂灵 赖金龙 杨旭 朱勇兵 张宇 赵三平 Yin Maoling;Lai Jinlong;Yang Xu;Zhu Yongbing;Zhang Yu;Zhao Sanping(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621000,China;State Key Laboratory of NBC Protection for Civilian,Beijing 102205,China)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621000 [2]国民核生化灾害防护国家重点实验室,北京102205

出　　处：《生态毒理学报》2024年第5期309-320,共12页Asian Journal of Ecotoxicology

基　　金：智强基金项目(ZQ2021-01);国家自然科学基金项目(22176222,22106182)。

摘　　要：2,4,6-三硝基甲苯(TNT)是1种硝基芳香族污染物,对人类和生态环境造成严重危害。全面了解微生物对TNT胁迫的细胞反应,对于开发合理的TNT修复技术是必要的。本研究以蜡样芽孢杆菌T4为研究对象,筛选细菌最适的生物刺激因子,分析外源营养物质刺激对细菌生长及TNT转化的影响。利用转录组和代谢组学技术,揭示微生物对TNT的降解和耐受机制。结果显示,该细菌的最适碳氮源分别为葡萄糖、酵母粉。当TNT浓度为100 mg·L^(-1)时,培养基中葡萄糖和酵母粉浓度分别为9.08 g·L^(-1)和0.94 g·L^(-1)。在泥浆反应体系中接种TNT降解菌,TNT的降解率达到96.91%~97.73%。此外,共鉴定到892个差异表达基因和139个差异代谢物。组学联合分析表明,氨基酸代谢、碳水化合物代谢、能量代谢和膜转运途径参与了细菌对TNT的降解及耐受过程。2,4,6-trinitrotoluene(TNT)poses severe hazards to humans and the environment as a nitroaromatic pollutant.A comprehensive understanding of how microorganisms respond to TNT stress at the cellular level is crucial for developing effective TNT remediation technologies.In this study,Bacillus cereus T4 was chosen to identify the most relevant biological stimulation factors for the bacteria and assess the impact of exogenous nutrients on bacterial growth and TNT transformation.Transcriptomic and metabolomic analyses uncovered TNT’s degradation and tolerance mechanisms under biologically stimulated conditions.They were the most suitable nutrient sources for this bacterial strain exposed to 100 mg·L^(-1)TNT,glucose(9.08 g·L^(-1)),and yeast extract(0.94 g·L^(-1)).In a slurry reaction system inoculated with the TNT-degrading bacterial strain,TNT degradation reached 96.91%~97.73%.Follow-up transcriptomics and metabolomics analysis identified 892 differentially expressed genes and 139 differentially expressed metabolites.Metabolic pathway analysis indicated that amino acid metabolism,carbohydrate metabolism,energy metabolism,and membrane transport were involved in this bacterial strain’s TNT degradation process.

关 键 词：2 4 6-三硝基甲苯 微生物修复 生物刺激 转录组学 代谢组学 

分 类 号：X171.5[环境科学与工程—环境科学]