二苯甲酮(BP-3)对树干毕赤酵母的细胞毒性和毒理  

Cytotoxicity and Toxicology of Benzophenone(BP-3)on Yeast Strain(Pichia stipitis)

作　　者：郑文丽 秦祎婷 胡双庆 赵晓祥[1] Zheng Wenli;Qin Yiting;Hu Shuangqing;Zhao Xiaoxiang(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China;Shanghai Academy of Environmental Sciences,Key Laboratory of Environmental Health Impact Assessment of New Pollutants for National Environmental Protection,Shanghai 200233,China)

机构地区：[1]东华大学环境科学与工程学院,上海201620 [2]上海市环境科学研究院国家环境保护新污染物环境健康影响评价重点实验室,上海200233

出　　处：《生态毒理学报》2024年第5期347-355,共9页Asian Journal of Ecotoxicology

基　　金：上海市生态环境局资助项目(沪环科2021-10)。

摘　　要：二苯甲酮(BP-3,氧苯酮)是1种常见的紫外线(UV)过滤剂,由于其生态毒理作用越来越受到关注。本研究目的是探索不同浓度的BP-3对树干毕赤酵母(Pichia stipitis)的毒性影响。将树干毕赤酵母分为溶剂对照组CK(二甲基亚砜DMSO)、CK(无菌水)、0.01、10、20、50、80和100 mg·L^(-1),从酵母细胞密度、细胞形态、细胞死亡率、细胞膜通透性、乙醇发酵、丙二醛(MDA)含量以及细胞抗氧化酶活性及抗氧化剂含量(超氧化物歧化酶SOD、过氧化物酶POD和过氧化氢酶CAT)进行检测。根据实验结果,揭示了BP-3对树干毕赤酵母的生态毒理效应。结果表明,不同浓度BP-3暴露后,树干毕赤酵母细胞生长受到抑制,死亡率随着暴露时间和BP-3浓度的增加而增加。从抗氧化指标来看,BP-3胁迫后,抗氧化酶活性受到一定影响。BP-3引起酵母细胞内活性氧(ROS)水平的增加,而脂质过氧化是由ROS引起的,ROS增加导致MDA含量增加。同时,为了消除过量ROS,胞内SOD和POD的活性随着BP-3浓度的增加而增加。BP-3具有亲脂性,通过破坏细胞膜进入细胞,导致DNA和蛋白质的泄露,同时也使细胞产乙醇能力下降。扫描电子显微镜(SEM)结果显示,BP-3会导致细胞表面产生皱纹和凹坑,导致细胞变形甚至破裂。这些发现为BP-3在生态环境中的风险评估提供了有价值的数据支撑。Benzophenone(BP-3,oxybenzone),a regularly used ultraviolet(UV)filter,is becoming a greater environmental issue due to its ecotoxicological effects.The aim of this study was to investigate the effects of different concentrations of BP-3 on the toxicity of yeast strain(Pichia stipitis).In the present study,yeast strain was divided into solvent control CK(dimethyl sulfoxide DMSO),CK(sterile water),0.01,10,20,50,80 and 100 mg·L^(-1) was analyzed in terms of yeast cell density,cell morphology,cell death rate,cell membrane permeability,ethanol fermentation,malondialdehyde(MDA)content as well as cellular antioxidant enzyme activity and antioxidant content(superoxide dismutase SOD,peroxidase POD and catalase CAT).Based on the experimental results,the ecotoxicological effects of BP-3 on yeast strains were revealed.The results showed that the growth of yeast strain cells was inhibited after exposure to different concentrations of BP-3,and the mortality rate increased with increasing exposure time and BP-3 concentration.Antioxidant enzyme activities were impacted by BP-3 stress in terms of antioxidant indices.Lipid peroxidation induced by BP-3 raised the intracellular concentration of reactive oxygen species(ROS),which in turn raised the levels of MDA.Meanwhile,to eliminate excess ROS,the activities of intracellular SOD and POD increased with increasing BP-3 concentration.Because BP-3 is lipophilic,it penetrates the cell by rupturing the cell membrane,which causes proteins and DNA to flow out and reduces the cell’s ability to produce ethanol.According to SEM,BP-3 causes wrinkles and pits on the cell surface,leading to cell deformation and even rupture.These results provide valuable data to support the risk assessment of BP-3 in ecosystems.

关 键 词：二苯甲酮 树干毕赤酵母 氧化应激 发酵 细胞形态 

分 类 号：X171.5[环境科学与工程—环境科学]