2种肉桂醛制剂对水产动物致病菌抑菌、杀菌效果比较  

Comparison of antibacterial and bactericidal effects of two cinnamaldehyde preparations on aquatic animal pathogens

作　　者：韩鑫怡 王庆奎 姚雨馨 韩妞妞 刘黎瑶 Han Xinyi;Wang Qingkui;Yao Yuxin;Han Niuniu;Liu Liyao(College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Key laboratory of Aqua-ecology and Aquaculture,Tianjin 300392,China;College of Basic Science,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院水产学院,天津300392 [2]天津市水产生态及养殖重点实验室,天津300392 [3]天津农学院基础科学学院,天津300392

出　　处：《天津农学院学报》2024年第5期67-71,共5页Journal of Tianjin Agricultural University

基　　金：国家级大学生创新创业项目(201910061003);国家重点研发计划(2020YFD0900600);现代农业产业技术体系专项资金(CARS-47);天津市海水养殖产业技术体系创新团队(ITTMRS2021000);天津市绿色生态饲料重点实验室开放基金(TJ202310);天津市水产生态及养殖重点实验室平台项目(无编号);水产生态与养殖国家级实验教学示范中心(天津农学院)(无编号)。

摘　　要：肉桂醛具有良好的抑菌、杀菌效果,但难溶于水、易氧化的特性限制了肉桂醛的应用范围。为提高肉桂醛水溶性并避免被氧化,本研究构建了纳米乳和羟丙基(β)环糊精包合物两种肉桂醛制剂,以链霉素为阳性对照,比较二者对创伤弧菌(Vibrio vulnificus)、哈维弧菌(Vibrio harveyi)、荧光假单胞菌(Pseudomonas fluorescens)、嗜水气单胞菌(Aeromonas hydrophila)、迟缓爱德华氏菌(Edwardsiella tarda)、温和气单胞菌(Aeromonas sobria)的抑菌、杀菌效果。结果表明:肉桂醛纳米乳制剂对上述6种水产致病菌的最小抑菌浓度(MIC)为0.93、2.05、1.67、14.88、9.30、1.86 mg/mL,最小杀菌浓度(MBC)为2.40、6.00、1.86、16.00、10.23、5.00 mg/mL,抑杀效果均大幅度优于肉桂醛-羟丙基(β)环糊精包合物。结论:与羟丙基(β)环糊精包合物相比,肉桂醛纳米乳制剂有更高的载药量,对致病菌有更强的抑菌、杀菌活力。虽然两种制剂对致病菌的抑菌、杀菌活力远低于链霉素,但是在绿色无抗水产养殖中仍具有潜在的应用前景。Cinnamaldehyde has good antibacterial,sterilization effect,but the features of being poorly soluble in water and easy oxidation limit its application range.In this study,cinnamaldehyde loaded nano-emulsion and hydroxypropyl(β)cyclodextrin inclusion were prepared.The determination of their antibacterial effect on 6 kinds of pathogenic bacteria-Vibrio vulnificus,Vibrio harveyi,Pseudomonas fluorescens,Aeromonas hydrophila,Edwardsiella tarda and Aeromonas sobria was applied with streptomycin as positive control.The results showed that the minimum inhibitory concentration(MIC)of cinnamaldehyde nano-emulsion against the six aquatic pathogenic bacteria mentioned above were 0.93,2.05,1.67,14.88,9.30,and 1.86 mg/mL,respectively.The minimum bactericidal concentration(MBC)were 2.40,6.00,1.86,16.00,10.23 and 5.00 mg/mL,respectively.The antibacterial effect of cinnamaldehyde nano-emulsion was significantly better than that of cinnamaldehyde hydroxypropyl(β)cyclodextrin inclusion.Conclusion:compared with cinnamaldehyde hydroxypropyl(β)cyclodextrin inclusion,cinnamaldehyde nano-emulsion has higher drug loading rate and better antibacterial activity against pathogenic bacteria.Although the antibacterial and bactericidal activity of the two formulations against pathogenic bacteria is much lower than that of streptomycin,they still have potential application prospects in aquaculture.

关 键 词：肉桂醛 纳米乳 最小杀菌浓度 最小抑菌浓度 水产致病菌 

分 类 号：S983[农业科学—捕捞与储运] S963.73[农业科学—水产科学]