虾青素通过调控氧化应激和炎症反应修复压力性损伤  

Astaxanthin Repairs Pressure Injury by Alleviating Oxidative Stress and Inflammation

作　　者：陈杨 马新润 王永辉 高蓓[2] 许贞蓉[2] 高艳虹[2] CHEN Yang;MA Xinrun;WANG Yonghui;GAO Bei;XU Zhenrong;GAO Yanhong(Department of Geriatrics,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200071,China;Department of Geriatrics,Xinhua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属第一人民医院老年医学科,上海200071 [2]上海交通大学医学院附属新华医院老年医学科,上海200092

出　　处：《医用生物力学》2024年第5期830-837,共8页Journal of Medical Biomechanics

基　　金：国家重点研发计划项目(2023YFC3605705);国家自然科学基金项目(82071575);上海交通大学医工交叉重点项目(YG2024ZD09);上海市科委基金项目(20ZR1435100)。

摘　　要：目的探究虾青素对小鼠压力性损伤创面的修复作用。方法体外实验:用不同浓度虾青素处理成纤维细胞,通过CCK-8实验检测细胞的增殖活性。随后通过缺氧/复氧诱导成纤维细胞损伤,给予最适浓度虾青素,用DHE荧光探针检测细胞内活性氧水平,RT-qPCR检测细胞内TNF-α、IL-1β、IL-6、IL-10、TGF-β的mRNA表达。体内实验:将两块圆形磁铁对称吸附于小鼠皮肤两侧,5 h后移除磁铁,以构建压力性损伤模型。随后分组灌胃等量的生理盐水、低剂量虾青素(10 mg/kg)与高剂量虾青素(20 mg/kg),定期采集创面图像。治疗7 d后,统计创面愈合率,并采集创面组织进行组织病理学染色。结果体外:应用虾青素后,缺氧/复氧损伤成纤维细胞内DHE荧光强度显著降低,TNF-α、IL-1β、IL-6 mRNA表达水平明显下调,TGF-β、IL-10 mRNA表达显著上调(P<0.05)。体内:高剂量虾青素组创面愈合率显著提高,DHE荧光强度显著降低,创面组织TNF-α、IL-6明显减少,TGF-β、IL-10明显增多(P<0.05)。结论虾青素可显著改善氧化应激,减轻炎症反应,对压力性损伤创面具有保护作用。Objective To explore the effects of astaxanthin on pressure injury wounds.Methods In vitro experiment:Fibroblasts were treated with different concentrations of astaxanthin and their proliferation activity was detected by CCK-8 assay.Subsequently,fibroblasts were induced by hypoxia/reoxygenation,and the optimal concentration of astaxanthin was administered.Then the intracellular reactive oxygen species(ROS)level was detected by DHE fluorescent probes and the mRNA expression level of TNF-α,IL-1β,IL-6,IL-10,TGF-βwas evaluated by RT qPCR.In vivo experiment:to construct a pressure injury model,two circular magnets were symmetrically adsorbed on both sides of the mouse skin for 5 hours everyday.Subsequently,equal amounts of physiological saline,low-dose astaxanthin(10 mg/kg),and high-dose astaxanthin(20 mg/kg)were administered by gavage in groups.Wound images were taken regularly.After 7-day treatment,wound healing rates were counted and wound tissues were collected for histopathological staining.Results In vitro,the fluorescence intensity of DHE in the astaxanthin groups were reduced dramatically.The relative mRNA expression level of TNF-α,IL-1β,IL-6 in the astaxanthin group declined,and the level of TGF-βand IL-10 mRNA increased significantly(P<0.05).In vivo,the wound healing rate and the level of TGF-β,IL-10 in high-dose astaxanthin group increased significantly.The ROS content and the level of TNF-α,IL-1βand IL-6 dropped markedly in astaxanthin groups(P<0.05).Conclusions Astaxanthin can significanlty alleviate oxidative stress,mitigate inflammation,thus exerting a protective effect on pressure injury wounds.

关 键 词：虾青素 抗氧化剂 压力性损伤 缺血-再灌注 缺氧/复氧损伤 

分 类 号：R318.01[医药卫生—生物医学工程]