超微粉碎枇杷枝条提取液的体外抗氧化活性及祛痰镇咳作用动物实验研究  

Study on the in vitro antioxidant activity and in vivo expectorant and antitussive effects of extracts from superfine loquat branches

作　　者：王若彤 温翠娟 袁娟 廖俊米 吕晓华[1] WANG Ruo-tong;WEN Cui-juan;YUAN Juan;LIAO Jun-mi;LYU Xiao-hua(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,四川成都610041 [2]北京市朝阳区疾病预防控制中心 [3]兽用功能性多肽制备国家地方联合工程研究中心

出　　处：《现代预防医学》2024年第20期3781-3786,共6页Modern Preventive Medicine

摘　　要：目的探究超微粉碎处理对枇杷枝条成分及功效的影响。方法枇杷枝条超微粉碎后,通过分光光度法和高效液相色谱法,检测不同粒径枇杷枝条提取液的总酚、总黄酮、多糖、熊果酸和齐墩果酸等功效成分含量。通过DPPH、ABTS自由基清除实验和FRAP实验,评价超微粉碎枇杷枝条提取液的体外抗氧化能力。将SPF级雄性昆明小鼠按体重随机分为空白/模型对照组、药物组、枇杷叶对照组、低、中、高剂量超微粉碎枇杷枝条组,利用酚红排泌实验评价超微粉碎枇杷枝条提取液的祛痰作用,利用氨水引咳实验评价超微粉碎枇杷枝条提取液的镇咳作用。结果三个粒径枇杷枝条组的总酚、总黄酮和齐墩果酸含量高于枝条对照组(P<0.05,P<0.01),220目和400目枇杷枝条组的总酚和总黄酮含量高于叶对照组(P<0.05),220目和400目枇杷枝条组的熊果酸含量高于枝条对照组(P<0.01)。体外实验中,三个粒径枇杷枝条组DPPH IC_(50)、ABTS IC_(50)均低于枝条对照组(P<0.05),FRAP值高于枝条对照组(P<0.05),200目和400目枇杷枝条组的DPPH IC_(50)、ABTS IC_(50)低于叶对照组(P<0.05),FRAP值高于叶对照组(P<0.05)。酚红排泌实验中,高剂量超微粉碎枇杷枝条组小鼠酚红排泌量显著提升(P<0.05)。氨水引咳实验中,中剂量和高剂量超微粉碎枇杷枝条组均能延长小鼠咳嗽的潜伏期和咳嗽次数(P<0.05)。结论适当的超微粉碎处理可提高枇杷枝条的功效成分含量,增强其抗氧化作用,同时具有一定祛痰镇咳功效。Objective To study the effects of superfine grinding treatment on the composition and efficacy of loquat branches.Methods After the loquat branches were superfine grinding treated,the contents of total phenols,total flavonoids,polysaccharides,ursolic acid,oleanolic acid in the extracts from loquat branches with different particle sizes were detected by spectrophotometry and high-performance liquid chromatography.The in vitro antioxidant capacity of extracts from superfine loquat branches was evaluated by DPPH,ABTS free radical scavenging assay and FRAP assay.SPF-grade male Kunming mice were randomly divided into blank/model control group,drug group,loquat leaf control group,and low,medium,and high dosage of superfine loquat branches according to their body weights.The expectorant effect of extracts from superfine loquat branches was evaluated by phenol red excretion assay,and the antitussive effect of extracts from superfine loquat branches was evaluated by ammonia-induced cough assay.Results The total phenol,total flavonoid and oleanolic acid contents of the three particle size loquat branch groups were higher than those of the branch control group(P<0.05,P<0.01),the total phenol and total flavonoid contents of the 220-mesh and 400-mesh loquat branch groups were higher than those of the leaf control group(P<0.05),and the ursolic acid contents of the 220-mesh and 400-mesh loquat branch groups were higher than those of the branch control group(P<0.01).In the in vitro experiment,the DPPH IC_(50) and ABTS IC_(50) of the three particle size loquat branch groups were lower than that of the branch control group(P<0.05),and the FRAP value was higher than that of the branch control group(P<0.05),and the DPPH IC_(50) and ABTS IC_(50) of the 200 mesh and 400 mesh loquat branch groups were lower than that of the leaf control group(P<0.05),and the FRAP value was higher than that of the leaf control group(P<0.05).In the phenol red excretion experiment,the phenol red excretion was significantly elevated in mice in the high-dos

关 键 词：枇杷枝条 超微粉碎 抗氧化 祛痰 镇咳 

分 类 号：R285.5[医药卫生—中药学]