蛋黄磷脂的毒理学安全性评价试验  

Evaluation on toxicological safety of egg yolk phospholipids

作　　者：刘礼泉 尹晓晨[2] 王蔚峰 胡余明[2] LIU Liquan;YIN Xiaochen;WANG Weifeng;HU Yuming(Hengyang Central Hospital,Hengyang,Hunan 421001,China;Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410153,China)

机构地区：[1]湖南省衡阳市中心医院,湖南衡阳421001 [2]湖南省疾病预防控制中心,湖南长沙410153

出　　处：《实用预防医学》2024年第8期929-936,共8页Practical Preventive Medicine

摘　　要：目的研究蛋黄磷脂的食用安全性,为该资源的高效开发利用提供科学依据。方法依据食品安全国家标准,对蛋黄磷脂进行毒理学安全性评价试验研究,包括急性经口毒性试验、三项遗传毒性试验、90 d经口毒性试验。结果急性经口毒性试验显示蛋黄磷脂为实际无毒级,三项遗传毒性试验显示结果均为阴性。90 d经口毒性试验期间,SD大鼠一般状况良好且无死亡;各剂量组雌、雄大鼠各时点体重、进食量、食物利用率及试验期间体重增长、总进食量、总食物利用率及血常规、血生化、凝血指标检测值与对照组比较差异无统计学意义(P>0.05);动物尿常规指标、眼部检查、大体解剖观察和病理组织学检查也未见与受试物有关的异常改变,大鼠90 d经口毒性试验提示蛋黄磷脂未对大鼠产生毒性作用,大鼠90 d经口毒性试验的未观察到有害作用剂量(no observed adverse effect level,NOAEL)为雄鼠8.03 g/kg·bw,雌鼠10.26 g/kg·bw。结论在该实验条件下,蛋黄磷脂未见急性毒性、遗传毒性及亚慢性毒性,具有较高的食用安全性。Objective To study the edible safety of egg yolk phospholipids,and to provide a scientific basis for the high-efficiency development and utilization of the resources.Methods According to the National Standard for Food Safety,we conducted an evaluation on toxicological tests of safety of egg yolk phospholipids,including acute oral toxicity test,three genetic toxicity tests and 90-day oral toxicity test.ResultsAcute oral toxicity test showed that egg yolk phospholipid was actually non-toxic grade,and three genetic toxicity tests displayed negative results.During the 90-day oral toxicity test,SD rats were in good condition and did not die.No statistically significant differences were found in body weight,food intake amount,food utilization rate,body weight gain during the tests,total food intake amount,total food utilization rate,blood routine,blood biochemical and coagulation indicators in female and male rats between each dose group and each control group(P>0.05).There were no abnormal changes related to tested substances in urine routine,ocular examination,gross anatomy and histopathology.The 90-day oral toxicity test revealed that egg yolk phospholipids did not have a toxic effect on rats.The dose of no observed adverse effect level in the 90-day oral toxicity test was 8.03 g/kg·bw for male rats and 10.26 g/kg·bw for female ones.Conclusion Under the experimental conditions,egg yolk phospholipids show no acute toxicity,genetic toxicity and sub-chronic toxicity,and thus have a high edible safety.

关 键 词：蛋黄磷脂 安全性评价试验 毒性研究 

分 类 号：R114[医药卫生—卫生毒理学]