灯笼草化学成分的分离和鉴定Ⅱ  

Isolation and identification of chemical constituents from Clinopodium polycephalum(II)

作　　者：李振兴 单雪梅 毕光明 刘亚轩 高源 孟大利[2] LI Zhenxing;SHAN Xuemei;BI Guangming;LIU Yaxuan;GAO Yuan;MENG Dali(Shandong Drug and Food Vocational College,Weihai 264210,China;School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]山东药品食品职业学院,山东威海264210 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2024年第10期1316-1322,共7页Journal of Shenyang Pharmaceutical University

基　　金：威海市中医药科技项目(2022N-22);山东药品食品职业学院院级课题(Y2022Y07);国家自然科学基金资助项目(81573694)。

摘　　要：目的对灯笼草(Clinopodium polycephalum)乙醇提取物进行化学成分研究.方法综合应用硅胶、ODS、大孔吸附树脂、Sephadex LH-20和高效液相色谱等方法对灯笼草乙醇提取物进行分离纯化,并根据理化性质及波谱数据对化合物进行结构鉴定.结果从灯笼草乙醇提取物中分离鉴定了15个化合物,分别为:松脂素(pinoresinol,1)、5′-甲氧基松脂素(medioresinol,2)、丁香树脂酚(syringaresinol,3)、1-羟基丁香树脂酚(1-hydroxysyringaresinol,4),4-酮松脂酚(4-ketopinoresinol,5)、(6R,7E,9R)-9-羟基-4,7-巨豆二烯-3-酮[(6R,7E,9R)-9-hydroxy-4-megastigmandien-3-one,6]、去氢吐叶醇(dehydrovomifoliol,7)、黑麦草内酯(loliolide,8)、芥子酸甲酯(methyl sinapate,9)、丁香酸甲酯(methyl syringate,10)、4-羟基肉桂酸甲酯(methyl 4-hydroxycinnamate,11)、醉鱼草皂苷Ⅳ(buddlejasaponin IV,12)、风轮菜皂苷D(clinoposaponin D,13)、异樱花苷(isosakuranin,14)、香蜂草苷(didymin,15).结论化合物1-5为首次从风轮菜属中分离得到.Objective To study the chemical constituents from Clinopodium polycephalum.Methods Multiple separation methods including macroporous adsorption resin chromatography,silica gel column chromatography,Sephadex LH-20 and HPLC method,were adopted to isolated compounds from the 70%ethanol extract of C.polycephalum and their structures were identified according to the physicochemical properties and spectral data.Results Fifteen compounds were identified as pinoresinol(1),medioresinol(2),syringaresinol(3),1-hydroxysyringaresinol(4),4-ketopinoresinol(5),(6R,7E,9R)-9-hydroxy-4-megastigmandien-3-one(6),dehydrovomifoliol(7),loliolide(8),methyl sinapate(9),methyl syringate(10)methyl 4-hydroxycinnamate(11),buddlejasaponin IV(12),clinoposaponin D(13),isosakuranin(14),didymin(15).Conclusion Compounds 1-5 were isolated from the genus Clinopodium for the first time.

关 键 词：风轮菜属 灯笼草 化学成分 分离鉴定 

分 类 号：R914[医药卫生—药物化学]