基于HPLC特征图谱和多成分含量评价梨干的质量  

Quality evaluation of Dried pear based on HPLC characteristic spectrum and multi-component content

作　　者：黄樱华[1,2] 赵巧慧 梁振生 骆月姬 HUANG Yinghua;ZHAO Qiaohui;LIANG Zhensheng;LUO Yueji(The first Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou 510405,China;School of Pharmaceutical Sciences,Guangzhou Universityof Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广东省中医临床研究院,广东广州510405 [3]广州中医药大学中药学院,广东广州510006

出　　处：《广东药科大学学报》2024年第5期79-86,共8页Journal of Guangdong Pharmaceutical University

基　　金：广州市科技计划项目(2023A03J0297);广东省岭南特色医院制剂转化工程技术研究中心(2023A170),粤科函产字〔2024〕346号。

摘　　要：目的建立梨干的特征图谱,测定其中熊果苷、绿原酸、芦丁3种成分的质量分数,结合化学模式识别方法,综合评价其质量差异。方法采用HPLC法,乙腈-0.1%(体积分数)磷酸水溶液作为流动相进行梯度洗脱,流速:1.0 mL/min;柱温:30℃;检测波长:0~25 min,280 nm;26~90 min,350 nm;进样量:20μL。采用“中药色谱特征图谱相似度评价系统(2012版)”软件,结合聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)综合评估20批梨干样本的HPLC特征图谱,同时测定其熊果苷、绿原酸、芦丁的质量分数。结果建立了20批梨干药材的特征图谱,相似度为0.959~0.997,标定了16个共有峰,同时指认了3个共有峰,分别为熊果苷、绿原酸、芦丁,其质量分数分别为16.78~66.00、47.69~260.10、1.39~5.48μg/g,绿原酸质量分数明显高于其他2种成分。通过HCA、PCA和OPLS-DA分析,把20批梨干分为2类(河北产地与非河北产地),并筛选出VIP值大于1的差异性标志成分6种,分别为峰9(绿原酸)、峰4、峰5、峰8、峰13、峰1(熊果苷)。结论本方法简便、准确,可用于梨干的质量控制。Objective To establish the HPLC fingerprints of Dried pear,determine the contents of Arbutin,Chlorogenic acid and Rutin in it,and comprehensively evaluate their quality differences by chemical pattern recognition methods.Methods HPLC was used,Acetonitrile-0.1%phosphoric acid was used as the mobile phase by gradient elution.The flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 280 nm(0~25 min)and 350 nm(26~90 min),and the injection amount was 20μL.Using fingerprint similarity evaluation software,cluster analysis(HCA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to comprehensively evaluate the HPLC characteristic spectra of 20 batches of dried pear samples.At the same time,the contents of arbutin,chlorogenic acid,and rutin were determined.Results The HPLC fingerprints of Dried pear were established with similarity ranging from 0.959 to 0.997.There were 16 common peaks in the fingerprints of Dried pears,and 3 common peaks were identified,which were Arbutin,Chlorogenic acid and Rutin,and the content determination results were 16.78-66.00μg/g,47.69-260.10μg/g,1.39-5.48μg/g,the content of Chlorogenic acid was much higher than Arbutin and Rutin.HCA,PCA and OPLS-DA analysis showed that 20 batches of Dried pears could be clustered into 2 classes(Hebei origin and Non Hebei origin),L2,L4,L5,L7 were clustered into one class(Hebei origin),and the rest were clustered into another class(Non Hebei origin).The VIP values of peak 9(Chlorogenic acid),peak4,peak5,peak8,peak13 and peak1(Arbutin)were greater than 1,which were differential marker components in Dried pear.Conclusion The HPLC fingerprints and content determination method established in this study are simple and accurate,which can be used for the quality control of Dried pear.

关 键 词：梨干 特征图谱 含量测定 化学模式识别法 

分 类 号：R284.1[医药卫生—中药学]