败酱草及其近缘种叶绿体全基因组分析和DNA条形码构建  

Chloroplast Whole Genome Analysis and DNA Barcode Construction of Patriniae Herba and Its Related Species

作　　者：孟文娜 浦香东 蒲婧哲 申传璞 陈庆 张磊[1] 吕雄文[1] 马陶陶[1] MENG Wen-na;PU Xiang-dong;PU Jing-zhe;SHEN Chuan-pu;CHEN Qing;ZHANG Lei;LYU Xiong-wen;MA Tao-tao(Traditional Chinese Medicine Formula Granule Center/Anhui Provincial Key Laboratory of Inflammation and Immunity,School of Pharmacy,Anhui Medical University,Hefei 230000,China;Key Laboratory for Research and Evaluation of Traditional Chinese Medicine Quality,Anhui Institute for Food and Drug Control,Hefei 230000,China;Bozhou Jiren Pharmaceutical,Bozhou 236800,China)

机构地区：[1]安徽医科大学药学院炎症免疫性疾病安徽省实验室/安徽医科大学中药配方颗粒中心,安徽合肥230000 [2]安徽省食品药品检验研究院中药质量研究与评价重点实验室,安徽合肥230000 [3]亳州济人药业,安徽亳州236800

出　　处：《中国现代中药》2024年第10期1645-1653,共9页Modern Chinese Medicine

基　　金：国家药品监督管理局中药质量研究与评价重点实验室开放课题(AHYJ-KFKT-202103)。

摘　　要：目的:基于败酱类药材基原植物的叶绿体基因组序列分析开发特定的DNA条形码,准确鉴别败酱草及其近缘种。方法:采用Illumina高通量测序技术对败酱科败酱属植物白花败酱、黄花败酱和异叶败酱的叶绿体基因组进行测序;采用生物信息学软件对基因组进行组装注释、特征分析、序列比较和系统发育分析;基于叶绿体基因组高突变区构建特异性DNA条形码进行验证。结果:3种败酱属植物叶绿体基因组呈四分体结构,全长分别为159585、158919、158919 bp,编码的基因数分别为130、132、132,包含8个rRNA基因和37个tRNA基因,蛋白质编码基因数分别为85、87、87。ccsA、ndhG、rpl23、ndhF序列作为特异性DNA条形码成功扩增16份样品,黄花败酱、白花败酱和少蕊败酱聚类在同一支,异叶败酱和糙叶败酱聚类在另一支。通过ccsA、ndhG、ndhF序列可进一步鉴别糙叶败酱和异叶败酱。结论:ccsA、ndhG、rpl23、ndhF序列可作为补充特异性DNA条形码区分败酱草及其近缘种。Objective:To develop specific DNA barcodes based on the chloroplast genome sequence analysis of the original plants of Patrinia medicinal materials to identify Patrniae Herba accurately and its related species.Methods:The chloroplast genomes of three species of the Patrinia plants,namely P.villosa,P.scabiosaefolia,and P.heterophylla,were sequenced using Illumina high-throughput sequencing technology.Bioinformatics software was employed for genome assembly,annotation,feature analysis,sequence comparison,and phylogenetic analysis.Specific DNA barcodes were constructed based on the highly variable regions of the chloroplast genome and were validated.Results:The chloroplast genomes of the three Patrinia plants exhibited a quadripartite structure,with total lengths of 159585 bp,158919 bp,and 158919 bp,respectively.They encoded 130,132,and 132 genes,including 8 rRNA genes and 37 tRNA genes.The number of protein-coding genes was 85,87,and 87,respectively.The ccsA,ndhG,rpl23,and ndhF sequences could be used as specific DNA barcodes,successfully amplifying 16 samples.P.scabiosaefolia,P.villosa,and P.monandra clustered together in the same branch,while P.heterophylla and P.scabra clustered in another branch.The ccsA,ndhG,and ndhF sequences further distinguished P.heterophylla from P.scabra.Conclusion:The sequences of ccsA,ndhG,rpl23,and ndhF can be used as supplementary specific DNA barcodes to distinguish Patriniae Herba and its related species.

关 键 词：败酱草 叶绿体基因组 分子鉴定 DNA条形码 系统发育分析 

分 类 号：R282.5[医药卫生—中药学]