绵羊肺炎支原体的分离鉴定及药物敏感性分析  

Isolation,identification and drug sensitivity of Mesomycoplasma ovipneumoniae

作　　者：刘芳[1] 乌志勇 王金玲[1] 苗苗 苏日娜[1] 白伟琴 卡楚拉 格日勒图[1] LIU Fang;WU Zhiyong;WANG Jinling;MIAO Miao;SU Rina;BAI Weiqin;Kachula;Geriletu(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《畜牧与兽医》2024年第11期109-113,共5页Animal Husbandry & Veterinary Medicine

基　　金：内蒙古自治区自然科学基金项目(2021MS03047);内蒙古农业大学兽医学院教学科技项目(SYKJYB202303)。

摘　　要：旨在通过收集陕西榆林市某羊场具有呼吸道病史的绵羊肺组织样本,开展病原微生物的分离鉴定及药敏试验。样本经培养后分离得到支原体疑似菌株,对可疑菌株进行形态学、生理生化鉴定,确定为疑似绵羊肺炎支原体(Mesomycoplasma ovipneumoniae)。通过16S rRNA特异性引物扩增获得361 bp目的基因片段。经测序后BLAST分析发现,分离菌株的基因片段均与绵羊肺炎支原体的Y98参考株(GenBank登录号:NR_025989.1)同源性为97.81%,在遗传进化树同一分支,将其命名为绵羊肺炎支原体SY-1菌株。按照梯度稀释SY-1菌液,采用解脲支原体(CCU)计数方法绘制其生长曲线,结果发现绵羊肺炎支原体的最高菌量可达到10^(9)CUU。药物敏感性试验结果显示,SY-1对多西环素高度敏感,对环丙沙星中度敏感,对头孢曲松、庆大霉素、苯唑西林、恩诺沙星和阿奇霉素均显示为低敏感度,而对利福平不敏感。本试验为羊场呼吸道疾病的防治和临床用药提供了参考。In this study,sheep lung tissue samples were collected from a sheep farm with a history of respiratory diseases;and isolation and identification of pathogenic microorganisms,and drug sensitivity tests were conducted.The samples were first purified and cultured through passage to obtain suspicious strains.Then,morphological,physiological,biochemical,and molecular biological identification of the suspicious strains of Mesomycoplasma ovipneumoniae was conducted.A 361 bp target gene fragment was amplified by PCR with 16S rRNA-specific primers.After sequencing,BLAST analysis revealed that the gene fragments of all three strains shared 97.81%homology with the M.ovipneumoniae Y98 reference strain(GenBank:NR_025989.1),which was named as M.ovipneumoniae SY-1 strain in the same branch of the genetic evolution tree.Finally,SY-1 bacterial solution was diluted in gradient and its growth curve was plotted using the CCU counting method.The results showed that the highest bacterial count of M.ovipneumoniae could reach 109 CUU.The drug sensitivity test results showed that SY-1 was highly sensitive to doxycycline,moderately sensitive to ciprofloxacin,and lowly sensitive to ceftriaxone,gentamicin,oxacillin,enrofloxacin,and azithromycin,but not sensitive to rifampicin.This study provided reference for the prevention and treatment of respiratory diseases in sheep farms and for clinical medication.

关 键 词：绵羊肺炎支原体 分离鉴定 遗传进化树 药物敏感性试验 

分 类 号：S852[农业科学—基础兽医学]