杜仲HSF基因家族的鉴定及其在逆境胁迫下的表达分析  

Identification of HSF Gene Family in Eucommia ulmoides and Its Expression Analysis under Adverse Stresses

作　　者：伍淑娟 徐枫 王光安[3] 王晨 WU Shujuan;XU Feng;WANG Guangan;WANG Chen(Puyang Higher Institute of Medical Sciences,Puyang 457000,China;Henan Tuina Vocational College,Luoyang 471023,China;Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China)

机构地区：[1]濮阳医学高等专科学校,河南濮阳457000 [2]河南推拿职业学院,河南洛阳471023 [3]河南中医药大学,河南郑州450008

出　　处：《河南农业科学》2024年第9期46-56,共11页Journal of Henan Agricultural Sciences

基　　金：河南中医药大学特色骨干学科顶目(STG-ZYX02-202114)。

摘　　要：热休克转录因子(Heat shock transcription factors,HSFs)在植物的生长发育和响应非生物胁迫过程中发挥着重要作用,为了解杜仲HSF基因家族成员信息,揭示其结构特征及表达模式,通过生物信息学方法和qRT-PCR对杜仲HSF基因家族的理化性质、蛋白质结构、系统进化、基因结构、保守域、启动子顺式作用元件及EuHSFs基因在杜仲叶片不同发育时期和不同非生物胁迫条件下的表达模式进行了分析。结果表明,从杜仲中共鉴定到21个EuHSFs基因,它们在蛋白质性质上存在差异,氨基酸数目、理论分子质量、等电点和不稳定系数分别介于68~369个、7.72~42.06 ku、4.31~9.22、7.05~67.28,且以酸性、亲水、不稳定的核蛋白为主。系统进化分析显示,EuHSFs被分成了3个亚组,包括ClassⅠ(1个)、ClassⅡ(7个)和ClassⅢ(13个)。通过启动子顺式作用元件分析发现,EuHSFs基因中存在大量的光响应元件和激素响应元件。EuHSFs基因在杜仲叶片不同发育时期均有表达,但表达模式存在显著差异。通过qRT-PCR检测发现,EuHSFs均能响应不同的非生物胁迫(高温、低温、高盐、干旱),如大部分EuHSFs在高温胁迫下随处理时间的延长表达量升高,且对高温和低温响应强烈。综上,杜仲HSF基因家族具有调控植物响应非生物胁迫的功能。Heat shock transcription factors(HSFs)play important roles in plant growth and development and in response to abiotic stresses.In order to understand the information of HSF gene family members in Eucommia ulmoides and reveal the structural characteristics and expression patterns of EuHSFs,this study analyzed the physicochemical properties,protein structure,phylogeny,gene structure,conserved domains,promoter cis⁃acting elements,and the expression patterns of EuHSF genes in different developmental periods and abiotic stresses by bioinformatics methods and qRT⁃PCR.The results showed that a total of 21 EuHSF genes were identified from Eucommia ulmoides,which differed in protein properties,with amino acid numbers,protein theoretical molecular masses,isoelectric points and instability coefficients ranging from 68—369,7.72—42.06 ku,4.31—9.22,and 7.05—67.28,respectively,and mainly consisted of acidic,hydrophilic,and unstable nuclear proteins.Phylogenetic analysis showed that EuHSFs were divided into three subgroups,including ClassⅠ(1 EuHSF),ClassⅡ(7 EuHSFs)and ClassⅢ(13 EuHSFs),and promoter cis⁃acting element analysis revealed that a large number of light⁃responsive and hormone⁃responsive elements in EuHSF genes.In addition,EuHSF genes were expressed at different developmental periods in Eucommia ulmoides leaves,but there were significant differences in the expression patterns.qRT⁃PCR revealed that all EuHSFs responded to different abiotic stresses(high temperature,low temperature,high salinity,and drought),e.g.,most of the EuHSFs showed elevated expression under high⁃temperature stress with the prolongation of the treatment time,and they were strongly responsive to both high and low temperatures.In summary,the EuHSF gene family has the function of regulating plant response to abiotic stresses.

关 键 词：杜仲 HSF 基因家族 系统进化 发育时期 非生物胁迫 表达模式 

分 类 号：S567.19[农业科学—中草药栽培]